The microbial composition of larval airways from Drosophila melanogaster differ between specimens from laboratory and natural habitats

Environmental Microbiome

Brief Report

Klaus F. Rabe¹  Birte Ehrhardt²  Christina Wagner²  Karin Uliczka²  Hanna Angstmann²  Susanne Krauss-Etschmann³  Thomas Roeder⁴  Susanne Kublik⁵  Stefan Pfeiffer⁶  Michael Schloter⁶ 

[1] Department of Pneumology, Lungen Clinic, Grosshansdorf, Germany;Department of Medicine, Christian Albrechts University, Germany Member of the German Center for Lung Research, Kiel, Germany;Division of Experimental Asthma Research, Early Life Origins of Chronic Lung Disease, Research Center Borstel, German Center for Lung Research (DZL), Airway Research Center North (ARCN), Leibniz Lung Center, Borstel, Germany;Division of Experimental Asthma Research, Early Life Origins of Chronic Lung Disease, Research Center Borstel, German Center for Lung Research (DZL), Airway Research Center North (ARCN), Leibniz Lung Center, Borstel, Germany;Department of Medicine, Institute for Experimental Medicine, Christian Albrechts University, Kiel, Germany;Division of Molecular Physiology, Institute of Zoology, Christian-Albrechts University, Airway Research Center North (ARCN), German Center for Lung Research (DZL), Kiel, Germany;Research Unit for Comparative Microbiome Analysis, Helmholtz Zentrum München, Oberschleißheim, Germany;ZIEL - Institute for Food and Health, Technical University of Munich, Freising, Germany;Research Unit for Comparative Microbiome Analysis, Helmholtz Zentrum München, Oberschleißheim, Germany;

关键词: Drosophila melanogaster;    Airway microbiome;    Intergenerational transmission;    Habitat dependent bacterial genera composition;    Immunodeficiency;   

DOI  :  10.1186/s40793-023-00506-9

 received in 2022-12-15, accepted in 2023-05-19,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundThe fruit fly Drosophila melanogaster lives in natural habitats and has also long been used as a model organism in biological research. In this study, we used a molecular barcoding approach to analyse the airways microbiome of larvae of D. melanogaster, which were obtained from eggs of flies of the laboratory strain w1118 and from immune deficient flies (NF-kB-K), and from wild-caught flies. To assess intergenerational transmission of microbes, all eggs were incubated under the same semi-sterile conditions.ResultsThe airway microbiome of larvae from both lab-strains was dominated by the two families Acetobacteraceae and Lactobacillaceae, while larvae from wild-caught flies were dominated by Lactobacillaceae, Anaplasmataceae and Leuconostocaceae. Barcodes linked to Anaplasmataceae could be further assigned to Wolbachia sp., which is a widespread intracellular pathogen in arthropods. For Leuconostoceae, the most abundant reads were assigned to Weissella sp. Both Wolbachia and Weissella affect the development of the insects. Finally, a relative high abundance of Serratia sp. was found in larvae from immune deficient relish−/− compared to w1118 and wild-caught fly airways.ConclusionsOur results show for the first time that larvae from D. melanogaster harbor an airway microbiome, which is of low complexity and strongly influenced by the environmental conditions and to a lesser extent by the immune status. Furthermore, our data indicate an intergenerational transmission of the microbiome as shaped by the environment.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

Fig. 12

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