Meta-analysis Reveals Potential Influence of Oxidative Stress on the Airway Microbiomes of Cystic Fibrosis Patients

Genomics, Proteomics & Bioinformatics

Zhancheng Gao¹  Yu Kang²  Jun Yu³  Liping Zhao³  Xing Shi³  Qin Ma⁴  Qiang Lin⁵ 

[1] University of Chinese Academy of Sciences, Beijing 100190, China;Bioinformatics and Mathematical Biosciences Lab, Department of Agronomy, Horticulture and Plant Science and Department of Mathematics and Statistics, South Dakota State University, Brookings, SD 57007, USA;CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China;Department of Biochemistry and Microbiology, School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA;Department of Respiratory & Critical Care Medicine, Peking University People’s Hospital, Beijing 100044, China;

关键词: Cystic fibrosis;    Airway microbiome;    Meta-analysis;    ROS;    Respiratory pathogens;   

DOI  :  

来源: DOAJ

【 摘 要 】

The lethal chronic airway infection of the cystic fibrosis (CF) patients is predisposed by colonization of specific CF-philic pathogens or the CF microbiomes, but key processes and reasons of the microbiome settlement in the patients are yet to be fully understood, especially their survival and metabolic dynamics from normal to diseased status under treatment. Here, we report our meta-analysis results on CF airway microbiomes based on metabolic networks reconstructed from genome information at species level. The microbiomes of CF patients appear to engage much more redox-related activities than those of controls, and by constructing a large dataset of anti-oxidative stress (anti-OS) genes, our quantitative evaluation of the anti-OS capacity of each bacterial species in the CF microbiomes confirms strong conservation of the anti-OS responses within genera and also shows that the CF pathogens have significantly higher anti-OS capacity than commensals and other typical respiratory pathogens. In addition, the anti-OS capacity of a relevant species correlates with its relative fitness for the airways of CF patients over that for the airways of controls. Moreover, the total anti-OS capacity of the respiratory microbiome of CF patients is collectively higher than that of controls, which increases with disease progression, especially after episodes of acute exacerbation and antibiotic treatment. According to these results, we propose that the increased OS in the airways of CF patients may play an important role in reshaping airway microbiomes to a more resistant status that favors the pre-infection colonization of the CF pathogens for a higher anti-OS capacity.

【 授权许可】

Unknown