Airway bacterial and fungal microbiome in chronic obstructive pulmonary disease

Medicine in Microecology

Hongwei Zhou¹  Zhenyu Liang²  Zhang Wang³  Zhiming Xiang³  Fengyan Wang⁴  Yan He⁵  Rongchang Chen⁶  Xilan Tan⁶  Xinzhu Yi⁷  Zuheng Liu⁷  Jin Su⁷  Haiyue Liu⁷  Yuqiong Yang⁸  Nannan Cao⁸  Chunxi Li⁸ 

[1] Corresponding author. Institute of Ecological Sciences, School of Life Sciences, South China Normal University, Guangzhou, China.;Department of Clinical Laboratory, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian, China;Chronic Airways Diseases Laboratory, Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China;Department of Cardiology, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian, China;Department of Radiology, Panyu Central Hospital of Guangzhou, Guangzhou, China;Institute of Ecological Science, School of Life Science, South China Normal University, Guangzhou, China;State Key Laboratory of Organ Failure Research, Microbiome Medicine Center, Division of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China;State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China;

关键词: COPD;    Frequent exacerbator;    Airway microbiome;    Mycobiome;    Bacterial-fungal interactions;   

DOI  :  

来源: DOAJ

【 摘 要 】

Little is known about airway mycobiome, and its relationship with bacterial microbiome in chronic obstructive pulmonary disease (COPD). Here we report the first simultaneous characterization of sputum bacterial and fungal microbiome in 84 stable COPD and 29 healthy subjects, using 16S ribosomal DNA and fungal internal transcribed spacer DNA sequencing. Ascomycota predominated over Basidiomycota in fungal microbiome both in COPD patients and healthy controls. Meyerozyma, Candida, Aspergillus and Schizophyllum were most abundant at the genus level. There was a significant inverse correlation between bacterial and fungal microbial diversity, both of which altered in opposite directions in COPD patients versus controls, and in frequent versus non-frequent exacerbators. An enhanced bacterial-fungal ecological interaction was observed in COPD patients, which was characterized by higher proportion of co-occurrence intrakingdom interactions and co-exclusive interkingdom interactions. In COPD, four mutually co-occurring fungal operational taxonomic units (OTUs) in Candida palmioleophila, Aspergillus and Sordariomycetes exhibited co-exclusive relationships with other fungal OTUs, which was specifically present in frequent exacerbators but not in non-frequent exacerbators. The perturbed bacterial-fungal interactions in COPD were associated with increased airway inflammatory mediators such as IL-6 and IL-8. The disruption of airway bacterial-fungal community balance, characterized by the loss of commensal bacterial taxa and enriched pathogenic fungal taxa, is implicated in COPD. The emergence of pathogenic fungi such as Candida and Aspergillus could be a marker for the frequent exacerbator phenotype. The airway mycobiome is an important cofactor mediating pathogenic infection and host inflammation in COPD.

【 授权许可】

Unknown