Weighted gene coexpression correlation network analysis reveals the potential molecular regulatory mechanism of citrate and anthocyanin accumulation between postharvest ‘Bingtangcheng’ and ‘Tarocco’ blood orange fruit

BMC Plant Biology

Research

Jian Han¹  Dazhi Li²  Huimin Zhang²  Manyu Liao²  Ling Sheng²  Yan Jin²  Junfeng Yang²  Na Li²  Xiaopeng Lu²  Ziniu Deng²  Xiaoqian Ma²  Guiyou Long² 

[1] Hunan Horticultural Research Institute, Changsha, CS, China;National Center for Citrus Improvement Changsha, College of Horticulture, Hunan Agricultural University, Changsha, CS, China;

关键词: Transcriptome;    Anthocyanin;    Citrate;    ‘Bingtangcheng’ sweet orange;    ‘Tarocco’ blood orange;    Postharvest;   

DOI  :  10.1186/s12870-023-04309-5

 received in 2023-04-05, accepted in 2023-05-23,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundOrganic acids and anthocyanins are the most important compounds for the flavor and nutritional quality of citrus fruit. However, there are few reports on the involvement of co-regulation of citrate and anthocyanin metabolism. Here, we performed a comparative transcriptome analysis to elucidate the genes and pathways involved in both citrate and anthocyanin accumulation in postharvest citrus fruit with ‘Tarocco’ blood orange (TBO; high accumulation) and ‘Bingtangcheng’ sweet orange (BTSO; low accumulation).ResultsA robust core set of 825 DEGs were found to be temporally associated with citrate and anthocyanin accumulation throughout the storage period through transcriptome analysis. Further according to the results of weighted gene coexpression correlation network analysis (WGCNA), the turquoise and brown module was highly positively correlated with both of the content of citrate and anthocyanin, and p-type ATPase (PH8), phosphoenolpyruvate carboxylase kinase (PEPCK), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), flavonoid 3’-hydroxylase (F3’H) and glutathione S transferase (GST) were considered key structural genes. Moreover, MYB family transcription factor (PH4), Zinc finger PHD-type transcription factor (CHR4, HAC12), Zinc finger SWIM-type transcription factor (FAR1) and Zinc finger C3H1-type transcription factor (ATC3H64) were considered hub genes related to these structural genes. Further qRT-PCR analysis verified that these transcription factors were highly expressed in TBO fruit and their expression profiles were significantly positively correlated with the structural genes of citrate and anthocyanin metabolism as well as the content of citrate and anthocyanin content.ConclusionsThe findings suggest that the CHR4, FAR1, ATC3H64 and HAC12 may be the new transcription regulators participate in controlling the level of citrate and anthocyanin in postharvest TBO fruit in addition to PH4. These results may providing new insight into the regulation mechanism of citrate and anthocyanin accumulation in citrus fruit.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

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