Response of anthocyanin biosynthesis to light by strand-specific transcriptome and miRNA analysis in Capsicum annuum

BMC Plant Biology

Yonghao Zhang¹  Zhuang Yang²  Jie Zhu²  Guopeng Zhu²  Wenlong Bao²  Yan Zhou²  Muhammad Ali Mumtaz²  Huangying Shu²  Zhiwei Wang²  Shanhan Cheng²  Yuanyuan Hao² 

[1] Institute of Tropical Horticulture Research in Hainan Academy of Agricultural Sciences, 571100, Haikou, China;Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province/Engineering Research Center of the Ministry of Education for New Variety Breeding of Tropical Crop, School of Horticulture, Hainan University, 570228, Haikou, China;

关键词: Pepper;    Anthocyanin;    Transcriptome;    lncRNA;    miRNA;    Photosensitivity;   

DOI  :  10.1186/s12870-021-03423-6

来源: Springer

【 摘 要 】

BackgroundAnthocyanins have distinct biological functions in plant coloring, plant defense against strong light, UV irradiation, and pathogen infection. Aromatic hydroxyl groups and ortho-dihydroxyl groups in anthocyanins are able to inhibit free-radical chain reactions and hydroxyl radicals. Thus, anthocyanins play an antioxidative role by removing various types of ROS. Pepper is one of the solanaceous vegetables with the largest cultivation area in China. The purple-fruited pepper is rich in anthocyanins, which not only increases the ornamental nature of the pepper fruit but also benefits the human body. In this experiment, light-induced regulatory pathways and related specific regulators of anthocyanin biosynthesis were examined through integrative transcriptomic and metabolomic analysis.ResultsResults revealed that delphinium 3-O-glucoside significantly accumulated in light exposed surface of pepper fruit after 48 h as compared to shaded surface. Furthermore, through strand-specific sequencing technology, 1341 differentially expressed genes, 172 differentially expressed lncRNAs, 8 differentially expressed circRNAs, and 28 differentially expressed miRNAs were identified significantly different among both surfaces. The flavonoid synthesis pathway was significantly enriched by KEGG analysis including SHT (XM_016684802.1), AT-like (XM_016704776.1), CCoAOMT (XM_016698340.1, XM_016698341.1), CHI (XM_016697794.1, XM_016697793.1), CHS2 (XM_016718139.1), CHS1B (XM_016710598.1), CYP98A2-like (XM_016688489.1), DFR (XM_016705224.1), F3’5’H (XM_016693437.1), F3H (XM_016705025.1), F3’M (XM_016707872.1), LDOX (XM_016712446.1), TCM (XM_016722116.1) and TCM-like (XM_016722117.1). Most of these significantly enriched flavonoid synthesis pathway genes may be also regulated by lncRNA. Some differentially expressed genes encoding transcription factors were also identified including MYB4-like (XM_016725242.1), MYB113-like (XM_016689220.1), MYB308-like (XM_016696983.1, XM_016702244.1), and EGL1 (XM_016711673.1). Three ‘lncRNA-miRNA-mRNA’ regulatory networks with sly-miR5303, stu-miR5303g, stu-miR7997a, and stu-miR7997c were constructed, including 28 differentially expressed mRNAs and 6 differentially expressed lncRNAs.ConclusionPossible light regulated anthocyanin biosynthesis and transport genes were identified by transcriptome analysis, and confirmed by qRT-PCR. These results provide important data for further understanding of the anthocyanin metabolism in response to light in pepper.

【 授权许可】

CC BY