Study of phenotypic and gene expression response to bacterial challenge

Behavior;Transcriptome;Functional Analysis

Nixon, Scott Evan

关键词: Behavior;    Transcriptome;    Functional Analysis;   

Others  :  https://www.ideals.illinois.edu/bitstream/handle/2142/78763/NIXON-DISSERTATION-2015.pdf?sequence=1&isAllowed=y

美国|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

In the mouse model of Bacillus Calmette-Guérin (BCG)-induced inflammation, focus is placed upon phenotypic and gene-expression responses to the original challenge. Studies of the phenotypic response to BCG involves several behavior indicators, categorized as representing an initial period of sickness response, and a set of depression-like behaviors that can endure for several weeks. Little is known about the impact of relationships among indicators on these studies, as most analyses have treated indicators independently. Gene-expression studies have also been small in scale, limiting what is known about gene-expression during these behavioral changes. With the availability of Next-Generation Sequencing platforms, the scale of transcriptome analysis can be greatly increased. This study aims to address these previous limitations, characterizing behavioral and gene-expression responses to BCG-induced inflammation.