Nano hydrogel-based oxygen-releasing stem cell transplantation system for treating diabetic foot

Journal of Nanobiotechnology

Research

Liangmiao Chen¹  Suifang Lin²  Yu Zhu²  Bingru Zheng²  Wanrui Wu²  Wei Cai²  Yizhou Xu²  Changsheng Shi²  Xiangpang Xie²  Ya Luo²  Changzheng Sun³ 

[1] Department of Endocrinology, The Third Affiliated Hospital of Wenzhou Medical University, 325200, Wenzhou, Zhejiang, China;Department of Interventional Vascular Surgery, The Third Affiliated Hospital of Wenzhou Medical University, No.108 Wansong Road, 325200, Wenzhou, Zhejiang, China;Institute of Cardiovascular Development and Translational Medicine, The Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, 325027, Wenzhou, Zhejiang, China;

关键词: Microspheres;    Stem cells;    Diabetic foot;    Hydrogel;    Transplantation;   

DOI  :  10.1186/s12951-023-01925-z

 received in 2023-03-23, accepted in 2023-05-10,  发布年份 2023

来源: Springer

【 摘 要 】

The employment of stem cells and hydrogel is widespread in contemporary clinical approaches to treating diabetic foot ulcers. However, the hypoxic conditions in the surrounding lesion tissue lead to a low stem cell survival rate following transplantation. This research introduces a novel hydrogel with superior oxygen permeability and biocompatibility, serving as a vehicle for developing a stem cell transplantation system incorporating oxygen-releasing microspheres and cardiosphere-derived stem cells (CDCs). By optimizing the peroxidase fixation quantity on the microsphere surface and the oxygen-releasing microsphere content within the transplantation system, intracellular oxygen levels were assessed using electron paramagnetic resonance (EPR) under simulated low-oxygen conditions in vitro. The expression of vascularization and repair-related indexes were evaluated via RT-PCR and ELISA. The microspheres were found to continuously release oxygen for three weeks within the transplantation system, promoting growth factor expression to maintain intracellular oxygen levels and support the survival and proliferation of CDCs. Moreover, the effect of this stem cell transplantation system on wound healing in a diabetic foot mice model was examined through an in vivo animal experiment. The oxygen-releasing microspheres within the transplantation system preserved the intracellular oxygen levels of CDCs in the hypoxic environment of injured tissues. By inhibiting the expression of inflammatory factors and stimulating the upregulation of pertinent growth factors, it improved the vascularization of ulcer tissue on the mice’s back and expedited the healing of the wound site. Overall, the stem cell transplantation system in this study, based on hydrogels containing CDCs and oxygen-releasing microspheres, offers a promising strategy for the clinical implementation of localized stem cell delivery to improve diabetic foot wound healing.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

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