Fanca deficiency is associated with alterations in osteoclastogenesis that are rescued by TNFα

Cell & Bioscience

Research

Frédérique Maczkowiak-Chartois¹  Filippo Rosselli¹  Lovely Monney¹  Alessia Oppezzo²  Lofti Slimani³  Henri Kilian³ 

[1] CNRS UMR9019, Équipe labellisée La Ligue contre le Cancer, Gustave Roussy Cancer Campus, 114 rue Edouard Vaillant, 94805, Villejuif, France;Gustave Roussy Cancer Center, Villejuif, France;Université Paris Saclay, Orsay, France;CNRS UMR9019, Équipe labellisée La Ligue contre le Cancer, Gustave Roussy Cancer Campus, 114 rue Edouard Vaillant, 94805, Villejuif, France;Gustave Roussy Cancer Center, Villejuif, France;Université Paris Saclay, Orsay, France;IFOM ETS, The AIRC Institute of Molecular Oncology, Milan, Italy;URP2496 Pathologies, Imagerie et Biothérapies Orofaciales et Plateforme Imagerie du Vivant (PIV), FHU-DDS-net, Dental School, Université de Paris, Montrouge, France;

关键词: Fanconi anemia;    Osteoclast;    Osteoblast;    Cell signaling;    TNFα;    p53;   

DOI  :  10.1186/s13578-023-01067-7

 received in 2023-02-21, accepted in 2023-06-09,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundHematopoietic stem cells (HSCs) reside in the bone marrow (BM) niche, which includes bone-forming and bone-resorbing cells, i.e., osteoblasts (OBs) and osteoclasts (OCs). OBs originate from mesenchymal progenitors, while OCs are derived from HSCs. Self-renewal, proliferation and differentiation of HSCs are under the control of regulatory signals generated by OBs and OCs within the BM niche. Consequently, OBs and OCs control both bone physiology and hematopoiesis. Since the human developmental and bone marrow failure genetic syndrome fanconi anemia (FA) presents with skeletal abnormalities, osteoporosis and HSC impairment, we wanted to test the hypothesis that the main pathological abnormalities of FA could be related to a defect in OC physiology and/or in bone homeostasis.ResultsWe revealed here that the intrinsic differentiation of OCs from a Fanca−/− mouse is impaired in vitro due to overactivation of the p53–p21 axis and defects in NF-kB signaling. The OC differentiation abnormalities observed in vitro were rescued by treating Fanca−/− cells with the p53 inhibitor pifithrin-α, by treatment with the proinflammatory cytokine TNFα or by coculturing them with Fanca-proficient or Fanca-deficient osteoblastic cells.ConclusionsOverall, our results highlight an unappreciated role of Fanca in OC differentiation that is potentially circumvented in vivo by the presence of OBs and TNFα in the BM niche.

【 授权许可】

CC BY   
© The Author(s) 2023

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