Genetic determinants of Biofilm formation of Helicobacter pylori using whole-genome sequencing

BMC Microbiology

Research

Vo Phuoc Tuan¹  Junko Akada²  Takashi Matsumoto²  Michiyuki Yurugi²  Kartika Afrida Fauzia³  Yoshio Yamaoka⁴  Ricky Indra Alfaray⁵  Evariste Tshibangu Kabamba⁶  Hafeza Aftab⁷  Phawinee Subsomwong⁸  Langgeng Agung Waskito⁹  Muhammad Miftahussurur^1,10 

[1] Department of Endoscopy, Cho Ray Hospital, 749000, Ho Chi Minh, Vietnam;Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, 879-5593, Yufu, Japan;Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, 879-5593, Yufu, Japan;Department of Public Health and Preventive Medicine, Faculty of Medicine, Universitas Airlangga, 60115, Surabaya, Indonesia;Helicobacter pylori and Microbiota Study Group, Institute of Tropical Disease, Universitas Airlangga, 60115, Surabaya, Indonesia;Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, 879-5593, Yufu, Japan;Division of Gastroentero-Hepatology, Department of Internal Medicine, Faculty of Medicine-Dr. Soetomo Teaching Hospital, Universitas Airlangga, 60115, Surabaya, Indonesia;Department of Medicine, Gastroenterology and Hepatology Section, Baylor College of Medicine, 77030, Houston, TX, USA;Borneo Medical and Health Research Centre, University Malaysia Sabah, 88400, Kota Kinabalu, Sabah, Malaysia;The Research Center for GLOBAL and LOCAL Infectious Diseases (RCGLID), Oita University, 879-5593, Yufu, Oita, Japan;Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, 879-5593, Yufu, Japan;Helicobacter pylori and Microbiota Study Group, Institute of Tropical Disease, Universitas Airlangga, 60115, Surabaya, Indonesia;Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, 879-5593, Yufu, Japan;Research Center for Infectious Sciences, Department of Parasitology, Graduate School of Medicine, Osaka City University, Osaka, Japan;Department of Gastroenterology, Dhaka Medical College and Hospital, 1000, Dhaka, Bangladesh;Department of Microbiology and Immunology, Hirosaki University Graduate School of Medicine, 036-8562, Hirosaki, Aomori, Japan;Helicobacter pylori and Microbiota Study Group, Institute of Tropical Disease, Universitas Airlangga, 60115, Surabaya, Indonesia;Department of Physiology and Biochemistry, Faculty of Medicine, Universitas Airlangga, 60115, Surabaya, Indonesia;Helicobacter pylori and Microbiota Study Group, Institute of Tropical Disease, Universitas Airlangga, 60115, Surabaya, Indonesia;Division of Gastroentero-Hepatology, Department of Internal Medicine, Faculty of Medicine-Dr. Soetomo Teaching Hospital, Universitas Airlangga, 60115, Surabaya, Indonesia;

关键词: Whole-genome sequences;    SNP;    Variants;    Biofilm formation;    Antibiotic resistance;    Infectious disease;   

DOI  :  10.1186/s12866-023-02889-8

 received in 2023-02-02, accepted in 2023-05-10,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundInfection with Helicobacter pylori as the cause of gastric cancer is a global public health concern. In addition to protecting germs from antibiotics, biofilms reduce the efficacy of H. pylori eradication therapy. The nucleotide polymorphisms (SNPs) related with the biofilm forming phenotype of Helicobacter pylori were studied.ResultsFifty-six H. pylori isolate from Bangladeshi patients were included in this cross-sectional study. Crystal violet assay was used to quantify biofilm amount, and the strains were classified into high- and low-biofilm formers As a result, strains were classified as 19.6% high- and 81.4% low-biofilm formers. These phenotypes were not related to specific clades in the phylogenetic analysis. The accessories genes associated with biofilm from whole-genome sequences were extracted and analysed, and SNPs among the previously reported biofilm-related genes were analysed. Biofilm formation was significantly associated with SNPs of alpA, alpB, cagE, cgt, csd4, csd5, futB, gluP, homD, and murF (P < 0.05). Among the SNPs reported in alpB, strains encoding the N156K, G160S, and A223V mutations were high-biofilm formers.ConclusionsThis study revealed the potential role of SNPs in biofilm formation and proposed a method to detect mutation in biofilm from whole-genome sequences.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

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