Molecular identification of non-tuberculous mycobacterial species isolated from extrapulmonary samples using real-time PCR and rpoB sequence analysis

AMB Express

Original Article

Atousa Ghorbani¹  Maryam Moradi Bandbal¹  Aram Asarehzadegan Dezfuli¹  Soolmaz Khandan Dezfuli¹  Mahtab Hamed²  Mohammad Hashemzadeh³  Azar Dokht Khosravi³ 

[1] Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran;Immunobiology Center of Pasteur Medical Laboratory, Ahvaz, Iran;Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran;Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran;

关键词: Nontuberculous mycobacteria;    Tuberculosis;    Extrapulmonary;    Pulmonary;    Molecular methods;   

DOI  :  10.1186/s13568-023-01553-8

 received in 2023-01-17, accepted in 2023-04-25,  发布年份 2023

来源: Springer

【 摘 要 】

Tuberculosis (TB) is one of the leading causes of mortality among infectious diseases and accounts for a serious health hazard wordwide. Apart from TB, the members of non-tuberculous mycobacteria (NTM), which includes around 170 species, may also cause different diseases in humans. Therefore this study aimed to investigate the distribution of NTM strains isolated from extrapulmonary (EP) samples by Real-Time PCR and PCR-sequencing methods in Southwest Iran. Three hundred and twenty-five suspected EP samples were collected from patients referred to the referral hospitals in Ahvaz, Iran. The isolates were initially screened by acid fast staining and identified by phenotypic culture and biochemical tests. The Real-Time PCR and rpoB- based PCR methods were performed followed by sequence analysis of rpoB gene. From 124 samples, 77 (62%) were positive for NTM by culture and rpoB sequence analysis. M. fortuitum was the most commonly isolated NTM in present study. In Real-Time PCR, only 69 (55.64%) isolates showed more homology with standard NTM isolates. In general, the growing trend of EPNTM infections in Iran needs specific programs and resources to get a better diagnosis. PCR sequencing is a reliable method, it can be used for definitive identification of positive cultures for identification of NTM species.

【 授权许可】

CC BY   
© The Author(s) 2023