Hippocampal Astrocyte Response to Melatonin Following Neural Damage Induction in Rats

Basic and Clinical Neuroscience

article

Abdolreza Babaee¹  Seyed Hassan Eftekhar Vaghefi¹  Samereh Dehghani Soltani²  Majid Asadi Shekaari³  Nader Shahrokhi³  Mohsen Basiri³ 

[1] Department of Anatomy, Afzalipour School of Medicine, Kerman University of Medical Sciences;Department of Anatomy, School of Medicine, Rafsanjan University of Medical Sciences;Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences

关键词: Melatonin;    Astrocyte;    GFAP;    Hippocampus;   

DOI  :  10.32598/bcn.12.2.986.1

来源: Iran University of Medical Sciences

【 摘 要 】

Introduction: Brain injury induces an almost immediate response from glial cells, especially astrocytes. Activation of astrocytes leads to the production of inflammatory cytokines and reactive oxygen species that may result in secondary neuronal damage. Melatonin is an anti-inflammatory and antioxidant agent, and it has been reported to exert neuroprotection through the prevention of neuronal death in several models of central nervous system injury. This study aimed to investigate the effect of melatonin on astrocyte activation induced by Traumatic Brain Injury (TBI) in rat hippocampus and dentate gyrus.  Methods: Animals were randomly divided into 5 groups; Sham group, TBI group, vehicle group, and melatonin‐treated TBI groups (TBI+Mel5, TBI+Mel20). Immunohistochemical method (GFAP marker) and TUNEL assay were used to evaluate astrocyte reactivity and neuronal death, respectively.  Results: The results demonstrated that the astrocyte number was reduced significantly in melatonin‐treated groups compared to the vehicle group. Additionally, based on TUNEL results, melatonin administration noticeably reduced the number of apoptotic neurons in the rat hippocampus and dentate gyrus.  Conclusion: In general, our findings suggest that melatonin treatment after brain injury reduces astrocyte reactivity as well as neuronal cell apoptosis in rat hippocampus and dentate gyrus.

【 授权许可】

Unknown