Development and characterization of 26 novel microsatellite loci for the trochid gastropod Gibbula divaricata (Linnaeus, 1758), using Illumina MiSeq next generation sequencing technology

PeerJ

article

Violeta López-Márquez¹  Ricardo García-Jiménez²  José Templado¹  Annie Machordom¹ 

[1] Biodiversidad y Biología Evolutiva;Molecular Systematics Lab

关键词: Microsatellite;    Genetic structure;    Population genetics;    Cross amplification;    Hybridization;    Gibbula varia;    Gibbula rarilineata;    Mediterranean sea;   

DOI  :  10.7717/peerj.1789

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

In the present study we used the high-throughput sequencing technology Illumina MiSeq to develop 26 polymorphic microsatellite loci for the marine snail Gibbula divaricata. Four to 32 alleles were detected per locus across 30 samples analyzed. Observed and expected heterozygosities ranged from 0.130 to 0.933 and from 0.294 to 0.956, respectively. No significant linkage disequilibrium existed. Seven loci deviated from Hardy-Weinberg equilibrium that could not totally be explained by the presence of null alleles. Sympatric distribution with other species of the genus Gibbula, as G. rarilineata and G. varia, lead us to test the cross utility of the developed markers in these two species, which could be useful to test common biogeographic patterns or potential hybridization phenomena, since morphological intermediate specimens were found.

【 授权许可】

CC BY