Genetic diversity and genetic structure of the Siberian roe deer (Capreolus pygargus) populations from Asia

BMC Genetics

Kyung Seok Kim³  Hang Lee¹  Mi-Sook Min¹  Yong-Su Park⁵  Jang Geun Oh⁶  Damdingiin Bayarlkhagva⁷  Alexander Argunov²  Inna Voloshina⁴  Nickolay Markov⁸  Yun Sun Lee¹ 

[1] Conservation Genome Resource Bank for Korean Wildlife, College of Veterinary Medicine, Seoul National University, Gwanak-gu 151-742, Seoul, Republic of Korea;Institute for Biological problems of Cryolihtozone Siberian Branch of Russian Academy of Sciences, Yakutsk 677980, Russia;Department of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames 50011, IA, USA;Lazovsky State Nature Reserve, Lazo 692980, Primorsky Krai, Russia;Department of Conservation Ecology, National Institute of Ecology, 1210, Geumgang-ro, Maseo-myeon, Seocheon-gun 325-813, Chungcheongnam-do, South Korea;Research Institute for Hallasan, Jeju Special Self-Governing Province, Jeju 690-815, Republic of Korea;Department of Molecular Biology and Genetics, National University of Mongolia, Ulaanbaatar 210646, Mongolia;Institute of Plant and Animal Ecology Urals Branch of Russian Academy of Sciences, Yekaterinburg 620144, Russia

关键词: Capreolus pygargus;    Siberian roe deer;    Genetic structure;    Genetic diversity;    Gene flow;    Microsatellite;   

Others  :  1223575
DOI  :  10.1186/s12863-015-0244-6

 received in 2015-02-02, accepted in 2015-06-29,  发布年份 2015

【 摘 要 】

Background

The roe deer, Capreolus sp., is one of the most widespread meso-mammals of Palearctic distribution, and includes two species, the European roe deer, C. capreolus inhabiting mainly Europe, and the Siberian roe deer, C. pygargus, distributed throughout continental Asia. Although there are a number of genetic studies concerning European roe deer, the Siberian roe deer has been studied less, and none of these studies use microsatellite markers. Natural processes have led to genetic structuring in wild populations. To understand how these factors have affected genetic structure and connectivity of Siberian roe deer, we investigated variability at 12 microsatellite loci for Siberian roe deer from ten localities in Asia.

Results

Moderate levels of genetic diversity (H_E= 0.522 to 0.628) were found in all populations except in Jeju Island, South Korea, where the diversity was lowest (H_E  = 0.386). Western populations showed relatively low genetic diversity and higher degrees of genetic differentiation compared with eastern populations (mean Ar = 3.54 (east), 2.81 (west), mean F_ST= 0.122). Bayesian-based clustering analysis revealed the existence of three genetically distinct groups (clusters) for Siberian roe deer, which comprise of the Southeastern group (Mainland Korea, Russian Far East, Trans-Baikal region and Northern part of Mongolia), Northwestern group (Western Siberia and Ural in Russia) and Jeju Island population. Genetic analyses including AMOVA (F_RT= 0.200), Barrier and PCA also supported genetic differentiation among regions separated primarily by major mountain ridges, suggesting that mountains played a role in the genetic differentiation of Siberian roe deer. On the other hand, genetic evidence also suggests an ongoing migration that may facilitate genetic admixture at the border areas between two groups.

Conclusions

Our results reveal an apparent pattern of genetic differentiation among populations inhabiting Asia, showing moderate levels of genetic diversity with an east-west gradient. The results suggest at least three distinct management units of roe deer in continental Asia, although genetic admixture is evident in some border areas. The insights obtained from this study shed light on management of Siberian roe deer in Asia and may be applied in conservation of local populations of Siberian roe deer.

【 授权许可】

   
2015 Lee et al.

【 图 表 】

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