Stress responses of the oil-producing green microalga Botryococcus braunii Race B

PeerJ

article

Ivette Cornejo-Corona¹  Hem R. Thapa²  Daniel R. Browne²  Timothy P. Devarenne²  Edmundo Lozoya-Gloria¹ 

[1] Genetic Engineering, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional;Department of Biochemistry and Biophysics, Texas A&M University, College Station

关键词: DAD1;    OST2;    PCD;    ROS;    Hydrocarbons;    NaCl;    NaHCO3;    Salicylic acid;    Methyl jasmonate;    Acetic acid;   

DOI  :  10.7717/peerj.2748

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Plants react to biotic and abiotic stresses with a variety of responses including the production of reactive oxygen species (ROS), which may result in programmed cell death (PCD). The mechanisms underlying ROS production and PCD have not been well studied in microalgae. Here, we analyzed ROS accumulation, biomass accumulation, and hydrocarbon production in the colony-forming green microalga Botryococcus braunii in response to several stress inducers such as NaCl, NaHCO3, salicylic acid (SA), methyl jasmonate, and acetic acid. We also identified and cloned a single cDNA for the B. braunii ortholog of the Arabidopsis gene defender against cell death 1 (DAD1), a gene that is directly involved in PCD regulation. The function of B. braunii DAD1 was assessed by a complementation assay of the yeast knockout line of the DAD1 ortholog, oligosaccharyl transferase 2. Additionally, we found that DAD1 transcription was induced in response to SA at short times. These results suggest that B. braunii responds to stresses by mechanisms similar to those in land plants and other  organisms.

【 授权许可】

CC BY