The anti-biofilm effect of silver-nanoparticle-decorated quercetin nanoparticles on a multi-drug resistant Escherichia coli strain isolated from a dairy cow with mastitis

PeerJ

article

Lumin Yu¹  Fei Shang¹  Xiaolin Chen¹  Jingtian Ni¹  Li Yu²  Ming Zhang¹  Dongdong Sun¹  Ting Xue¹ 

[1] School of Life Sciences, Anhui Agricultural University;Department of Microbiology and Parasitology, Anhui Key Laboratory of Zoonoses, Anhui Medical University

关键词: Biofilm;    Bovine mastitis;    Escherichia coli;    Quercetin;    Silver nanoparticles;   

DOI  :  10.7717/peerj.5711

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundEscherichia coli is an important opportunistic pathogen that could cause inflammation of the udder in dairy cows resulting in reduced milk production and changes in milk composition and quality, and even death of dairy cows. Therefore, mastitis is the main health issue which leads to major economic losses on dairy farms. Antibiotics are routinely used for the treatment of bovine mastitis. The ability to form biofilm increases the antibiotic resistance of E. coli. Nanoparticles (NPs), a nanosized, safe, and highly cost-effective antibacterial agent, are potential biomedical tools. Given their antibacterial activities, silver nanoparticles (Ag NPs) have a broad range of applications.MethodsIn this study, we performed antibacterial activity assays, biofilm formation assays, scanning electron microscopy (SEM) experiments, and real-time reverse transcription PCR (RT-PCR) experiments to investigate the antibacterial and anti-biofilm effect of quercetin, Ag NPs, and Silver-nanoparticle-decorated quercetin nanoparticles (QA NPs) in E. coli strain ECDCM1.ResultsIn this study, QA NPs, a composite material combining Ag NPs and the plant-derived drug component quercetin, exhibited stronger antibacterial and anti-biofilm properties in a multi-drug resistant E. coli strain isolated from a dairy cow with mastitis, compared to Ag NPs and Qe.DiscussionThis study provides evidence that QA NPs possess high antibacterial and anti-biofilm activities. They proved to be more effective than Ag NPs and Qe against the biofilm formation of a multi-drug resistant E. coli isolated from cows with mastitis. This suggests that QA NPs might be used as a potential antimicrobial agent in the treatment of bovine mastitis caused by E. coli.

【 授权许可】

CC BY