Didymin improves UV irradiation resistance in C. elegans

PeerJ

article

Lin Zhou¹  Lu Wang¹  Jialing Zhang¹  Jiahe Li¹  Shuju Bai¹  Junfeng Ma¹  Xueqi Fu¹ 

[1] National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin Province

关键词: Didymin;    Caenorhabditis elegans;    Lifespan;    UV irradiation;   

DOI  :  10.7717/peerj.6218

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Didymin, a type of flavono-o-glycoside compound naturally present in citrus fruits, has been reported to be an effective anticancer agent. However, its effects on stress resistance are unclear. In this study, we treated Caenorhabditis elegans with didymin at several concentrations. We found that didymin reduced the effects of UV stressor on nematodes by decreasing reactive oxygen species levels and increasing superoxide dismutase (SOD) activity. Furthermore, we found that specific didymin-treated mutant nematodes daf-16(mu86) & daf-2(e1370), daf-16(mu86), akt-1(ok525), akt-2(ok393), and age-1(hx546) were susceptible to UV irradiation, whereas daf-2(e1371) was resistant to UV irradiation. In addition, we found that didymin not only promoted DAF-16 to transfer from cytoplasm to nucleus, but also increased both protein and mRNA expression levels of SOD-3 and HSP-16.2 after UV irradiation. Our results show that didymin affects UV irradiation resistance and it may act on daf-2 to regulate downstream genes through the insulin/IGF-1-like signaling pathway.

【 授权许可】

CC BY