期刊论文详细信息
PeerJ
Characterization of aminopeptidase encoding gene anp-1 and its association with development in Caenorhabditis elegans
article
Shanchun Su1  Baoliang Pan1  Yanxin Hu1  Ming Wang1 
[1] Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University;Institute of Anesthesiology and Pain ,(IAP), Taihe Hospital
关键词: Caenorhabditis elegans;    Aminopeptidases;    Lifespan;    Body size;   
DOI  :  10.7717/peerj.7944
学科分类:社会科学、人文和艺术(综合)
来源: Inra
PDF
【 摘 要 】

Background Aminopeptidases play important roles in various biological processes in nematodes including growth, development and reproduction. Although the aminopeptidases have been shown to regulate reproduction in Caenorhabditis elegans (C. elegans), the role of aminopeptidases in development and aging has not been reported. This study focused on the function of aminopeptidase AlaNyl aminopeptidase 1 (ANP-1) on development in C. elegans. Methods In the present study, we reported the identification of ANP-1 in C. elegans along with sequence analysis and its functional expression and characterization. The phenotype changes were observed when anp-1 mutated. Then, differential expression genes (DEGs) between wild type strain (N2) and anp-1 deletion strain (RB804) were identified using transcriptome sequencing method. Finally, DEGs were verified by qRT-PCR assay. Results Our observations suggested that anp-1 mutation induced small body size in the L4/young adult stage of C. elegans, however, there was no difference between N2 and RB804 in adult stage. Moreover, deletion of anp-1 resulted in shortening lifespan and laying fewer eggs. DEGs (184 genes) were observed between N2 groups and RB804 groups by transcriptome sequencing. According to GO annotations and KEGG enrichment analysis, these DEGs play vital roles in development regulation in C. elegans. These data demonstrate ANP-1 participates in development and aging of C. elegans and will considerably contribute to the existing knowledge of aminopeptidase function in C. elegans.

【 授权许可】

CC BY   

【 预 览 】
附件列表
Files Size Format View
RO202307100009353ZK.pdf 14609KB PDF download
  文献评价指标  
  下载次数:14次 浏览次数:4次