Identification, expression, and phylogenetic analyses of terpenoid biosynthesis-related genes in secondary xylem of loblolly pine ( Pinus taeda L.) based on transcriptome analyses

PeerJ

article

Jipeng Mao¹  Zidi He¹  Jing Hao¹  Tianyi Liu¹  Jiehu Chen²  Shaowei Huang¹ 

[1] College of Forestry and Landscape Architecture, South China Agricultural University;Science Corporation of Gene

关键词: Loblolly pine (Pinus taeda L.);    Transcriptome analysis;    Terpenoid biosynthesis;    Phylogenetic analysis;    Conserved motifs;   

DOI  :  10.7717/peerj.6124

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Loblolly pine (Pinus taeda L.) is one of the most important species for oleoresin (a mixture of terpenoids) in South China. The high oleoresin content of loblolly pine is associated with resistance to bark beetles and other economic benefits. In this study, we conducted transcriptome analyses of loblolly pine secondary xylem to gain insight into the genes involved in terpenoid biosynthesis. A total of 372 unigenes were identified as being critical for oleoresin production, including genes for ATP-binding cassette (ABC) transporters, the cytochrome P450 (CYP) protein family, and terpenoid backbone biosynthesis enzymes. Six key genes involved in terpenoid biosynthetic pathways were selected for multiple sequence alignment, conserved motif prediction, and phylogenetic and expression profile analyses. The protein sequences of all six genes exhibited a higher degree of sequence conservation, and upstream genes were relatively more conserved than downstream genes in terpenoid biosynthetic pathways. The N-terminal regions of these sequences were less conserved than the C-terminal ends, as the N-terminals were quite diverse in both length and composition. The phylogenetic analyses revealed that most genes originated from gene duplication after species divergence, and partial genes exhibited incomplete lineage sorting. In addition, the expression profile analyses showed that all six genes exhibited high expression levels during the high-oleoresin-yielding phase.

【 授权许可】

CC BY