Evidence for an oncogenic role of HOXC6 in human non-small cell lung cancer

PeerJ

article

Yingcheng Yang¹  Xiaoping Tang¹  Xueqin Song¹  Li Tang¹  Yong Cao¹  Xu Liu¹  Xiaoyan Wang¹  Yan Li¹  Minglan Yu¹  Haisu Wan¹  Feng Chen¹ 

[1] Experimental Medicine Center, The Affiliated Hospital of Southwest Medical University

关键词: HOXC6;    Non-small cell lung cancer;    Molecular marker;    Bioinformatics;    Malignancy;   

DOI  :  10.7717/peerj.6629

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundIdentification of specific biomarkers is important for the diagnosis and treatment of non-small cell lung cancer (NSCLC). HOXC6 is a homeodomain-containing transcription factor that is highly expressed in several human cancers; however, its role in NSCLC remains unknown.MethodsThe expression and protein levels of HOXC6 were assessed in NSCLC tissue samples by Quantitative real-time PCR (qRT-PCR) and immunohistochemistry, respectively. HOXC6 was transfected into the NSCLC cell lines A549 and PC9, and used to investigate its effect on proliferation, migration, and invasion using CFSE, wound healing, and Matrigel invasion assays. Next-generation sequencing was also used to identify downstream targets of HOXC6 and to gain insights into the molecular mechanisms underlying its biological function.ResultsHOXC6 expression was significantly increased in 66.6% (20/30) of NSCLC tumor samples in comparison to normal controls. HOXC6 promoted proliferation, migration, and invasion of NSCLC cells in vitro. RNA-seq analysis demonstrated the upregulation of 310 and 112 genes in A549-HOXC6 and PC9-HOXC6 cells, respectively, and the downregulation of 665 and 385 genes in A549-HOXC6 and PC9-HOXC6 cells, respectively. HOXC6 was also found to regulate the expression of genes such as CEACAM6, SPARC, WNT6, CST1, MMP2, and KRT13, which have documented pro-tumorigenic functions.DiscussionHOXC6 is highly expressed in NSCLC, and it may enhance lung cancer progression by regulating the expression of pro-tumorigenic genes involved in proliferation, migration, and invasion. Our study highlighted the oncogenic potential of HOXC6, and suggests that it may be a novel biomarker for the diagnosis and treatment of NSCLC.

【 授权许可】

CC BY