RAPD primer screening for amplification on Katokkon pepper from Toraja, South Sulawesi, Indonesia

1st Biennial Conference on Tropical Biodiversity

Larekeng, S.H.^1 ; Dermawan, R.^2 ; Iswoyo, H.^2 ; Mustari, K.^2

Department of Forestry, Faculty of Forestry, Hasanuddin University, Jalan Perintis Kemerdekaan KM 10, Makassar, South Sulawesi

90245, Indonesia^1

Department of Agrotechnology, Faculty of Agriculture, Hasanuddin University, Jalan Perintis Kemerdekaan KM 10, Makassar, South Sulawesi

90245, Indonesia^2

关键词: Chinense jacq;    DNA samples;    Genetic diversity;    Indonesia;    Molecular marker;    Polymorphic bands;    Random amplified polymorphic dnas;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/270/1/012023/pdf
DOI  :  10.1088/1755-1315/270/1/012023

来源: IOP

【 摘 要 】

Random amplified polymorphic DNA (RAPD) is a well-known molecular marker that extensively applied to the genetic diversity analysis among species in the populations. Here, this marker was utilized in Katokkon pepper (Capsicum chinense Jacq.), a highland pepper and having unique characteristics cultivated in specific regions (Tana Toraja and North Toraja), on preliminary primer screening using 30 RAPD primer pairs. A total of eight primers (OPA-02, OPAE-11, OPG-09, M-29, OPP-08, OPG-19, OPZ-05, and OPD-20) could produce polymorphic bands on twelve randomly selected DNA samples. OPA-02, OPP-08, OPG-19, OPZ-05, and OPD-20 generated polymorphic bands on all twelve evaluated samples, yet OPZ-05 was excluded in further analysis due to producing unclear bands. Clear and polymorphic bands were formed by OPAE-11 on eleven samples, whilst OPG-09 and M-29 only produced bands on ten samples. The primers are suitable to be used for amplifying DNA and determining the genetic diversity of Katokkon pepper in further analyses.