Comprehensive analysis of prognostic value and immune infiltration of kindlin family members in non-small cell lung cancer

BMC Medical Genomics

Yuan Xu¹  Yiming Zeng¹  Xiaoshan Su¹  Weijing Wu¹  Zhixing Zhu²  Feng He³  Ning Liu³  Xinfu Chen³ 

[1] Department of Pulmonary and Critical Care Medicine, the Second Affiliated Hospital of Fujian Medical University, Respirology Medicine Centre of Fujian Province, Quanzhou, China;Department of Pulmonary and Critical Care Medicine, the Second Affiliated Hospital of Fujian Medical University, Respirology Medicine Centre of Fujian Province, Quanzhou, China;Department of Pathology and Biomedical Science, University of Otago, Christchurch, New Zealand;Department of Thoracic Surgery, Fuzhou Pulmonary Hospital, Fuzhou, China;

关键词: Kindlins;    Non-small cell lung cancer;    Bioinformatics;    Prognosis;   

DOI  :  10.1186/s12920-021-00967-2

来源: Springer

【 摘 要 】

BackgroundKindlin Family Members have been reported to be aberrantly expressed in various human cancer types and involved in tumorigenesis, tumor progression, and chemoresistance. However, their roles in non-small cell lung cancer (NSCLC) remain poorly elucidated.MethodsWe analyzed the prognostic value and immune infiltration of Kindlins in NSCLC through Oncomine, GEPIA, UALCAN, CCLE, Kaplan‑Meier plotter, cBioPortal, TIMER, GeneMANIA, STRING, and DAVID database. Additionally, the mRNA expression levels of Kindlins were verified in 30 paired NSCLC tissues and NSCLC cell lines by real-time PCR.ResultsThe expression level of FERMT1 was remarkably increased in NSCLC tissues and NSCLC cell lines, while FERMT2 and FERMT3 were reduced. Kindlins expressions were associated with individual cancer stages and nodal metastasis. We also found that higher expression level of FERMT1 was obviously correlated with worse overall survival (OS) in patients with NSCLC, while higher FERMT2 was strongly associated with better overall survival (OS) and first progression (FP). Additionally, the expression of FERMT2 and FERMT3 were obviously correlated with the immune infiltration of diverse immune cells. Functional enrichment analysis has shown that Kindlins may be significantly correlated with intracellular signal transduction, ATP binding and the PI3K-Akt signaling pathway in NSCLC.ConclusionsThe research provides a new perspective on the distinct roles of Kindlins in NSCLC and likely has important implications for future novel biomarkers and therapeutic targets in NSCLC.

【 授权许可】

CC BY