Description and classification of bivalve mollusks hemocytes: a computational approach

PeerJ

article

Yuriy A. Karetin¹  Aleksandra A. Kalitnik¹  Alina E. Safonova²  Eduardas Cicinskas² 

[1] National Scientific Center of Marine Biology of Far Eastern Branch of the Russian Academy of Sciences;Far Eastern Federal University

关键词: Hemocytes;    Morphometry;    Bivalve;    Fractal analysis;    Cell morphology;   

DOI  :  10.7717/peerj.7056

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

The fractal formalism in combination with linear image analysis enables statistically significant description and classification of “irregular” (in terms of Euclidean geometry) shapes, such as, outlines of in vitro flattened cells. We developed an optimal model for classifying bivalve Spisula sachalinensis and Callista brevisiphonata immune cells, based on evaluating their linear and non-linear morphological features: size characteristics (area, perimeter), various parameters of cell bounding circle, convex hull, cell symmetry, roundness, and a number of fractal dimensions and lacunarities evaluating the spatial complexity of cells. Proposed classification model is based on Ward’s clustering method, loaded with highest multimodality index factors. This classification scheme groups cells into three morphological types, which can be distinguished both visually and by several linear and quasi-fractal parameters.

【 授权许可】

CC BY