Morphometry of cellular behavior of coelomocytes from starfish Asterias amurensis

PeerJ

article

Yuriy A. Karetin¹ 

[1] A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences

关键词: Morphometry;    Asterias Amurensis;    Fractal analysis;    Classification;    Coelomocytes;   

DOI  :  10.7717/peerj.12514

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

A comprehensive statistical analysis using a wide range of linear and non-linear morphological parameters enabled identification of the main stages in the in vitro dynamics of cell behavior of immune cells of the marine invertebrate Asterias amurensis (Echinodermata, Asteroidea). Three stages may be distinguished in the cell behavior, which are characterized by the differences in complexity of the cell boundary microsculpture as well as by the size and asymmetry of the cell and convex hull of the cell. The first stage (5 min after placing cells onto a substrate) is characterized by more complex cell morphology and an increase in the process number and spreading area. The second stage (15 min) is characterized by simplification of cell morphology, retraction of some processes, and rounding of cells upon continued cell spreading. At the third stage (60 min), new large processes with rounded contours emerge due to partial retraction of the flattened cell surface. Each stage is characterized by statistically significant differences in several linear and nonlinear parameters of the external morphology for all cell types.

【 授权许可】

CC BY