Ultrastructural and quantitative studies of hemocytes in the sugarcane borer, Diatraea saccharalis (Lepidoptera: Pyralidae)

Brazilian Archives of Biology and Technology

Ângela Maria Ferreira Falleiros¹  Maria Terezinha Siqueira Bombonato²  Elisa Aparecida Gregório² 

[1] ,Universidade Estadual de Londrina Centro de Ciências Biológicas Departamento de HistologiaLondrina PR ,Brazil

关键词: Insect;    Lepidoptera;    Hemocytes;    Ultrastructural Morphology;    Differential Hemocyte Count;    Morphometry;   

DOI  :  10.1590/S1516-89132003000200021

来源: SciELO

【 摘 要 】

Six circulating hemocytes cell types from Diatraea saccharalis (Fabricius) (Lepidoptera: Pyralidae) larvae were identified by transmission and scanning electron microscope: prohemocytes (PR), plasmatocytes (PL) granulocytes (GR), spherulocytes (SP), oenocytoids (OE) and vermicytes (VE). The PR was the smallest cell type with a large nucleus, a cytoplasm with few organelles and a homogenous smooth surface. The PL was polymorphic and abundant, with a cytoplasm rich in organelles and a cellular surface with several cytoplasmic projections. The GR was abundant, showing two types of membrane-bounded granules (dense and structutered), glycogen, lipid droplets and a surface with philopodial projections. The SP was a large cell, with a cytoplasm full of intracytoplasmic spherules. The OE was the largest hemocyte type with a large and homogeneous cytoplasm and scarce organelles. The VE was discoid in shape and showed electron-dense granules.

【 授权许可】

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