PeerJ | |
Comparative transcriptomic and proteomic analyses of the green and white parts of chimeric leaves in Ananas comosus var. bracteatus | |
article | |
Yanbin Xue1  Jun Ma1  Yehua He2  Sanmiao Yu1  Zhen Lin1  Yingyuan Xiong1  Fatima Rafique1  Fuxing Jiang1  Lingxia Sun1  Mingdong Ma1  Yujue Zhou1  Xi Li1  Zhuo Huang1  | |
[1] College of Landscape Architecture, Sichuan Agricultural University;Horticultural Biotechnology College of South China Agricultural University, South China Agricultural University | |
关键词: Molecular mechanism; Chimeric leaves; Ananas comosus var. bracteatus; Transcriptome; Proteome; | |
DOI : 10.7717/peerj.7261 | |
学科分类:社会科学、人文和艺术(综合) | |
来源: Inra | |
【 摘 要 】
BackgroundAnanas comosus var. bracteatus has high ornamental value due to its chimeric leaves. However, the chimeric trait is very unstable in red pineapple plants, and transcriptional variation between the two types of cells (white/green cells) and the molecular mechanism responsible for their albino phenotype remain poorly understood.MethodsComparative transcriptomic and proteomic analyses of the white parts (Whs) and green parts (Grs) of chimeric leaves were performed.ResultsIn total, 1,685 differentially expressed genes (DEGs) (712 upregulated and 973 downregulated) and 1,813 differentially abundant proteins (DAPs) (1,018 with low abundance and 795 with high abundance) were identified. Based on Gene Ontology (Go) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses, the DEGs were mostly involved in carbon fixation in photosynthetic organisms, porphyrin and chlorophyll metabolism and oxidative phosphorylation, while proteomic analysis revealed that DAPs were mostly related to ribosomes, photosynthesis, photosynthesis antennas, and porphyrin and chlorophyll metabolism. Combined analysis showed increased mRNA levels but low abundance of nine proteins level in Whs /Grs related to photosynthetic pigment and photosynthesis. Transcriptional changes, posttranscriptional regulation and translational alterations of key enzymes involved in chlorophyll biosynthesis and photosynthesis may play important roles in the albino parts of chimeric leaves.
【 授权许可】
CC BY
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202307100010114ZK.pdf | 13082KB | download |