Differential proteomic analysis of replanted Rehmannia glutinosa roots by iTRAQ reveals molecular mechanisms for formation of replant disease

BMC Plant Biology

Research Article

Yanhui Yang¹  Junyi Zhang²  Li Gu³  Fajie Feng³  Chuyun Yang³  Bao Zhang³  Aiguo Chen³  Shuqiang Chen³  Mingjie Li³  Wenxiong Lin⁴  Zhongyi Zhang⁴  Xinjian Chen⁵  Fengqing Wang⁵ 

[1] College of Bioengineering, Henan University of Technology, Zhengzhou, China;College of Chemical Engineering, Huaqiao University, Xiamen, China;College of Crop Sciences, Fujian Agriculture and Forestry University, Fuzhou, China;College of Crop Sciences, Fujian Agriculture and Forestry University, Fuzhou, China;Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry University, Fuzhou, China;Henan Agricultural University, Zhengzhou, China;

关键词: R. glutinosa;    Transcriptome;    Proteins;    iTRAQ;    Replant disease;    Molecular mechanism;   

DOI  :  10.1186/s12870-017-1060-0

 received in 2016-11-18, accepted in 2017-06-22,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThe normal growth of Rehmannia glutinosa, a widely used medicinal plant in China, is severely disturbed by replant disease. The formation of replant disease commonly involves interactions among plants, allelochemicals and microbes; however, these relationships remain largely unclear. As a result, no effective measures are currently available to treat replant disease.ResultsIn this study, an integrated R. glutinosa transcriptome was constructed, from which an R. glutinosa protein library was obtained. iTRAQ technology was then used to investigate changes in the proteins in replanted R. glutinosa roots, and the proteins that were expressed in response to replant disease were identified. An integrated R. glutinosa transcriptome from different developmental stages of replanted and normal-growth R. glutinosa produced 65,659 transcripts, which were accurately translated into 47,818 proteins. Using this resource, a set of 189 proteins was found to be significantly differentially expressed between normal-growth and replanted R. glutinosa. Of the proteins that were significantly upregulated in replanted R. glutinosa, most were related to metabolism, immune responses, ROS generation, programmed cell death, ER stress, and lignin synthesis.ConclusionsBy integrating these key events and the results of previous studies on replant disease formation, a new picture of the damaging mechanisms that cause replant disease stress emerged. Replant disease altered the metabolic balance of R. glutinosa, activated immune defence systems, increased levels of ROS and antioxidant enzymes, and initiated the processes of cell death and senescence in replanted R. glutinosa. Additionally, lignin deposition in R. glutinosa roots that was caused by replanting significantly inhibited tuberous root formation. These key processes provide important insights into the underlying mechanisms leading to the formation of replant disease and also for the subsequent development of new control measures to improve production and quality of replanted plants.

【 授权许可】

CC BY   
© The Author(s). 2017