Development of genomic simple sequence repeat markers for Glycyrrhiza lepidota and cross-amplification of other Glycyrrhiza species

PeerJ

article

Chi Eun Hong¹  Sebastin Raveendar²  Kyong Hwan Bang¹  Kyung Ho Ma¹  Soon Wook Kwon³  Hojin Ryu⁴  Ick Hyun Jo¹  Jong-Wook Chung⁵  Jun Hyoung Bang⁵ 

[1] Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science;National Agrobiodiversity Center, National Institute of Agricultural Science;Department of Plant Bioscience, Pusan National University;Department of Biology, Chungbuk National University;Department of Industrial Plant Science and Technology, Chungbuk National University

关键词: Cross-amplification;    Genetic diversity;    Licorice;    Simple sequence repeat marker;    Transferability;   

DOI  :  10.7717/peerj.7479

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundLicorice (Glycyrrhiza spp. L.) is used as a natural sweetener and medicinal herb in European and Asian countries. Molecular studies have been conducted to find differences between wild and cultivated species because most wild species are highly resistant to abiotic and biotic stresses compared with their cultivated species. However, few molecular markers have been developed for studying the genetic diversity and population structure of licorice species and to identify differences between cultivars. Thus, the present study aimed to develop a set of genomic simple sequence repeat (SSR) markers for molecular studies of these species.MethodsIn the present study, we developed polymorphic SSR markers based on whole-genomesequence data of Glycyrrhiza lepidota. Then, based on the sequence information, the polymorphic SSR markers were developed. The SSR markers were applied to 23 Glycyrrhiza individual plants. We also evaluated the phylogenetic relationships and interspecies transferability among samples.ResultsThe genetic diversity analysis using these markers identified 2–23 alleles, and the major allele frequency, observed heterozygosity, genetic diversity, and polymorphism information content were 0.11–0.91, 0–0.90, 0.17–0.94, and 0.15–0.93, respectively. Interspecies transferability values were 93.5%, 91.6%, and 91.1% for G. echinata, G. glabra, and G. uralensis, respectively. Phylogenetic analysis clustered cultivated (group 1) and wild (group 2) species into three and two subgroups, respectively. The reported markers represent a valuable resource for the genetic characteri z ation of Glycyrrhiza spp. for theanalysis of its genetic variability, and as a tool for licorice transferability. This is the first intraspecific study in a collection of Glycyrrhiza spp. germplasm using SSR markers.

【 授权许可】

CC BY