Microsatellite markers for the Jaera albifrons species complex (marine isopods)

BMC Research Notes

Claire Daguin-Thiébaut¹  Thomas Broquet¹  Ambre Ribardière¹ 

[1] CNRS, Station Biologique de Roscoff, Sorbonne Universités, UPMC Univ Paris 06, UMR 7144, Team Diversity and Connectivity of Coastal Marine Landscapes, Roscoff, 29680, France

关键词: Multiplex PCR;    454 pyrosequencing;    Cross-amplification;    Species complex;    SSR;   

Others  :  1232866
DOI  :  10.1186/s13104-015-1595-9

 received in 2015-05-11, accepted in 2015-10-19,  发布年份 2015

【 摘 要 】

Background

The Jaera albifrons complex contains five species of marine isopods (J. albifrons, J. praehirsuta, J. ischiosetosa, J. forsmani, and J. posthirsuta). These species, occurring on the shores of the North-Atlantic Ocean, are partially reproductively isolated by barriers due to sexual isolation (mate choice), genetic incompatibilities, and ecological specialization. Microsatellite loci would be useful for parentage-based analyses of sexual selection and studies of genetic structure in the context of speciation.

Findings

Twenty-four microsatellite markers were developed for J. albifrons using pyrosequencing of enriched libraries. Patterns of polymorphisms were analyzed in 49 J. albifrons adult males sampled in two populations from Brittany (Western France). The average number of alleles per locus was 4.73 ± 2.45 and the average gene diversity was 0.55 ± 0.23. Most markers also successfully amplified in the three sibling species J. praehirsuta, J. ischiosetosa, and J. forsmani.

Conclusions

These polymorphic and cross-amplifiable markers will be useful for population genetics and parentage studies in the J albifrons complex.

【 授权许可】

   
2015 Ribardière et al.

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