Antimicrobial photodynamic therapy on Staphylococcus aureus and Escherichia coli using malachite green encapsulated mesoporous silica nanoparticles: an in vitro study

PeerJ

article

Parasuraman Paramanantham¹  Busi Siddhardha¹  Sruthil Lal SB²  Alok Sharan²  Abdullah A. Alyousef³  Mohammed Saeed Al Dosary³  Mohammed Arshad³  Asad Syed⁴ 

[1] Department of Microbiology, School of Life Sciences, Pondicherry University;Department of Physics, School of Physical, Chemical and Applied Sciences, Pondicherry University;Microbiology Research Group, Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University;Department of Botany and Microbiology, College of Science, King Saud University

关键词: aPDT;    Biofilms;    CLSM;    Mesoporous silica nanoparticle;    MG-MSN;   

DOI  :  10.7717/peerj.7454

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundRise in the number of healthcare associated or hospital acquired infections is a major problem affecting the global healthcare sector. We evaluated superior antibacterial and antibiofilm photodynamic therapy (aPDT) using malachite green encapsulated mesoporous silica nanoparticles (MG-MSN) against Staphylococcus aureus and Escherichia coli, which are known to be major causative agents of nosocomial infections.MethodsMalachite green (MG) was encapsulated on mesoporous silica nanoparticles (MSN). Fourier-transform infrared spectroscopy, Transmission electron microscopy, and spectroscopic analysis were performed to characterize the MG-MSN. The antimicrobial efficacies of MSN, MG, and MG-MSN were investigated and the results were recorded.ResultsMG-MSN was effective against both the tested bacteria. S. aureus was more phototoxic to MG-MSN compared to E. coli. The antibiofilm efficacy of MG-MSN on E. coli and S. aureus was also studied. Biofilm inhibition was 65.68 ± 2.62% in E. coli and 79.66 ± 3.82% in S. aureus. Cell viability assay, exopolysaccharides quantification, and confocal laser scanning microscopy studies also revealed the enhanced antibiofilm activity of MG-MSN when used as a potential photosensitizer for aPDT. This study can be extended to eradicate these strains from localized superficial infections and medical appliances, preventing nosocomial infections.

【 授权许可】

CC BY