Antimicrobial photodynamic therapy on Staphylococcus aureus and Escherichia coli using malachite green encapsulated mesoporous silica nanoparticles: an in vitro study

PeerJ

Asad Syed¹  Parasuraman Paramanantham²  Busi Siddhardha²  Sruthil Lal SB³  Alok Sharan³  Mohammed Saeed Al Dosary⁴  Mohammed Arshad⁴  Abdullah A. Alyousef⁴ 

[1] Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia;Department of Microbiology, School of Life Sciences, Pondicherry University, Pondicherry, India;Department of Physics, School of Physical, Chemical and Applied Sciences, Pondicherry University,Pondicherry, India;Microbiology Research Group, Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia;

关键词: aPDT;    Biofilms;    CLSM;    Mesoporous silica nanoparticle;    MG-MSN;   

DOI  :  10.7717/peerj.7454

来源: DOAJ

【 摘 要 】

Background Rise in the number of healthcare associated or hospital acquired infections is a major problem affecting the global healthcare sector. We evaluated superior antibacterial and antibiofilm photodynamic therapy (aPDT) using malachite green encapsulated mesoporous silica nanoparticles (MG-MSN) against Staphylococcus aureus and Escherichia coli, which are known to be major causative agents of nosocomial infections. Methods Malachite green (MG) was encapsulated on mesoporous silica nanoparticles (MSN). Fourier-transform infrared spectroscopy, Transmission electron microscopy, and spectroscopic analysis were performed to characterize the MG-MSN. The antimicrobial efficacies of MSN, MG, and MG-MSN were investigated and the results were recorded. Results MG-MSN was effective against both the tested bacteria. S. aureus was more phototoxic to MG-MSN compared to E. coli. The antibiofilm efficacy of MG-MSN on E. coli and S. aureus was also studied. Biofilm inhibition was 65.68 ± 2.62% in E. coli and 79.66 ± 3.82% in S. aureus. Cell viability assay, exopolysaccharides quantification, and confocal laser scanning microscopy studies also revealed the enhanced antibiofilm activity of MG-MSN when used as a potential photosensitizer for aPDT. This study can be extended to eradicate these strains from localized superficial infections and medical appliances, preventing nosocomial infections.

【 授权许可】

Unknown