Overexpression of PheNAC3 from moso bamboo promotes leaf senescence and enhances abiotic stress tolerance in Arabidopsis

PeerJ

article

Lihua Xie¹  Miaomiao Cai¹  Xiangyu Li¹  Huifang Zheng¹  Yali Xie¹  Zhanchao Cheng¹  Yucong Bai¹  Juan Li¹  Shaohua Mu¹  Jian Gao¹ 

[1] Key Laboratory of Bamboo and Rattan Science and Technology, International Center for Bamboo and Rattan, State Forestry and Grassland Administration;Pingdingshan University

关键词: NAC transcription factors;    Senescence;    Abiotic stress;    Moso bamboo;    Overexpression;    Transgenic Arabidopsis;    Transcriptional activity;    Subcellular localization;    NAM domain;    Phylogenetic analysis;   

DOI  :  10.7717/peerj.8716

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

The NAC family is one of the largest transcription factor families unique to plants, which regulates the growth and development, biotic and abiotic stress responses, and maturation and senescence in plants. In this study, PheNAC3, a NAC gene, was isolated and characterized from moso bamboo (Phyllostachys edulis). PheNAC3 belong to the NAC1 subgroup and has a conserved NAC domain on the N-terminus, which with 88.74% similarity to ONAC011 protein. PheNAC3 localized in the nucleus and exhibited transactivation activity. PheNAC3 was upregulated during the process of senescence of leaves and detected shoots. PheNAC3 was also induced by ABA, MeJA, NaCl and darkness, but it had no remarkable response to PEG and SA treatments. Overexpression of PheNAC3 could cause precocious senescence in Arabidopsis. Transgenic Arabidopsis displayed faster seed germination, better seedling growth, and a higher survival rate than the wild-type under salt or drought stress conditions. Moreover, AtSAG12 associated with senescence and AtRD29A and AtRD29b related to ABA were upregulated by PheNAC3 overexpression, but AtCAB was inhibited. These findings show that PheNAC3 may participate in leaf senescence and play critical roles in the salt and drought stress response.

【 授权许可】

CC BY