Identification of innovative potential quality markers in rocket and melon fresh-cut produce

卷:188

Cavaiuolo, Marina ; Cocetta, Giacomo ; Bulgari, Roberta ; Spinardi, Anna ; Ferrante, Antonio

关键词: Abiotic stress;    Fresh-cut vegetables;    Postharvest;    Production chain;    Senescence;   

DOI  :  10.1016/j.foodchem.2015.04.143

学科分类：食品科学和技术

【 摘 要 】

Ready-to-eat fresh cut produce are exposed to pre- and postharvest abiotic stresses during the production chain. Our work aimed to identify stress responsive genes as new molecular markers of quality that can be widely applied to leaves and fruits and easily determined at any stage of the production chain. Stress responsive genes associated with quality losses were isolated in rocket and melon fresh-cut produce and their expression levels analyzed by quantitative real time PCR (qRT-PCR) at different time points after harvest at 20 degrees C and 4 degrees C. qRT-PCR results were supported by correlation analysis with physiological and biochemical determinations evaluated at the same conditions such as chlorophyll a fluorescence indices, total, reducing sugars, sucrose, ethylene, ascorbic acid, lipid peroxidation and reactive oxygen species. In both species the putative molecular markers increased their expression soon after harvest suggesting a possible use as novel and objective quality markers of fresh-cut produces. (C) 2015 Elsevier Ltd. All rights reserved.