Antifungal activity of 8-methoxynaphthalen-1-ol isolated from the endophytic fungus Diatrype palmicola MFLUCC 17-0313 against the plant pathogenic fungus Athelia rolfsii on tomatoes

PeerJ

article

Chutima Tanapichatsakul¹  Acharavadee Pansanit¹  Sakon Monggoot²  Siraprapa Brooks¹  Surasak Prachya³  Prasat Kittakoop³  Parinya Panuwet⁶  Patcharee Pripdeevech¹ 

[1] School of Science, Mae Fah Luang University;Institute of Beauty and Health Sciences Co.,Ltd.;Chulabhorn Research Institute;Program in Chemical Sciences, Chulabhorn Royal Academy, Chulabhorn Graduate Institute;Center of Excellence on Environmental Health and Toxicology ,(EHT), CHE, Ministry of Education;Laboratory of Exposure Assessment and Development for Environmental Research, Department of Environmental Health, Rollins School of Public Health, Emory University;Center of Chemical Innovation for Sustainability ,(CIS), Mae Fah Luang University

关键词: Antifungal activity;    8-methoxynaphthalen-1-ol;    Endophytic fungi;    Plant pathogen;    Tomato;    Southern blight;    Diatrype;    Athelia rolfsii;   

DOI  :  10.7717/peerj.9103

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Thirty-four endophytic fungal isolates were obtained from the leaves of the medicinal plant Polyscias fruticosa, and their antagonistic activities against the growth of the common tomatoes plant pathogenic fungus Athelia rolfsii were initially screened using a dual culture assay. The endophytic fungus MFLUCC 17-0313, which was later molecularly identified as Diatrype palmicola, displayed the highest inhibition percentage (49.98%) in comparison to the others. This fungus was then chosen for further evaluation. Its culture broth and mycelia from a 10 L scale were separated and extracted using ethyl acetate, methanol, and hexane. Each extract was tested for antifungal activity against the same pathogen using a disc diffusion assay. Only the crude hexane extract of fungal mycelium showed antifungal activity. The hexane extract was fractioned using sephadex gel filtration chromatography and each fraction was tested for antifungal activity until the one with the highest inhibition percentage was obtained. The bioactive compound was identified as 8-methoxynaphthalen-1-ol using nuclear magnetic resonance spectroscopy and mass spectrometry. The minimum inhibition concentration of 8-methoxynaphthalen-1-ol was demonstrated at 250 µg/mL against the selected pathogen. Using the leaf assay, the solution of 8-methoxynapthalen-1-ol was tested for phytotoxic activity against A. rolfsii and was found to have no phytotoxic effects. These results showed that 8-methoxynaphthalen-1-ol has the potential for controlling the growth of A. rolfsii, the cause of Southern blight disease on tomatoes. This study may provide the foundation for future use of this compound as a biofungicide.

【 授权许可】

CC BY