Investigation of acute-phase proteins and cytokines response in goats with contagious caprine pleuropneumonia with special reference to their diagnostic accuracy

PeerJ

article

Wael El-Deeb¹  Mahmoud Fayez³  Ibrahim Elsohaby⁵  Mohamed Salem¹  Abdulrhman Alhaider¹  Mahmoud Kandeel⁸ 

[1] Clinical Sciences, College of Veterinary Medicine, King Faisal University;Department of Internal Medicine, Infectious Diseases and Fish Diseases, Faculty of Veterinary Medicine, Mansoura University;Department of Bacteriology, Veterinary Serum and Vaccine Research Institute, Ministry of Agriculture;Bacteriology, Al Ahsa Veterinary Diagnostic Laboratory;Department of Animal Medicine, Faculty of Veterinary Medicine, Zagazig University;Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island;Department of Medicine and Infectious Diseases, Faulty of Veterinary Medicine, Cairo University;Department of Biomedical Sciences, King Faisal University

关键词: C-reactive protein;    Procalcitonin;    Haptoglobin;    Serum amyloid a;    Cytokines;   

DOI  :  10.7717/peerj.10394

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Acute-phase proteins (APPs) have always had valued diagnostic potentialities in response to infection. This study aimed to evaluate the diagnostic accuracy of selected APPs and proinflammatory cytokines (PIC) in goats with contagious caprine pleuropneumonia (CCPP) under field conditions. Moreover, to highlight the role of tested biomarkers in CCPP pathogenesis. Fifty-eight goats (38 confirmed cases with CCPP and 20 healthy controls) were involved in this investigation. C-reactive protein (CRP), procalcitonin (PCT), haptoglobin (HP), fibrinogen (Fb), serum amyloid A (SAA), selected PIC (IL1-α, IL1-β, IL-6, interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α)) levels were investigated in serum samples from all goats under investigation. Latex agglutination test was used for diagnosis of goats with CCPP. For microbiological investigations, nasopharyngeal swabs (from all goats), lung tissues and pleural fluids (from only necropsied goats) were collected. This study revealed that all tested parameters have a high to moderate degree of diagnostic performance for CCPP. Magnitudes of increase in levels of APPs (CRP, HP and SAA) were stronger than PIC, IFN-γ90%), except HP (AUROC = 87.3%) and IFN-γ (AUROC = 78.8%) showed moderate accuracy in differentiation of goats with and without CCPP infection. For detecting goats with and without CCPP infection, HP had the lowest sensitivity (Se = 81.6%) and Fb had the lowest specificity (Sp = 85.0%) among the APPs parameters tested. However, PCT showed the highest Se (100%) and Sp (95.0%) to detect goats with and without CCPP infection among tested parameters. Conclusively, this study endorses the significance of selected APPs and PIC as additional screening diagnostic parameters for naturally occurring CCPP in goats. However, it does not replace traditional methods for diagnosis of CCPP in goats. Furthermore, APPs and PIC have an important role in disease pathogenesis in goats.

【 授权许可】

CC BY