Genotyping tools and resources to assess peanut germplasm: smut-resistant landraces as a case study

PeerJ

article

Alicia N. Massa¹  Marina Bressano²  Juan H. Soave³  Mario I. Buteler³  Guillermo Seijo⁴  Victor S. Sobolev¹  Valerie A. Orner¹  Claudio Oddino³  Sara J. Soave³  Paola C. Faustinelli¹  Francisco J. de Blas⁵  Marshall C. Lamb¹  Renee S. Arias¹ 

[1] National Peanut Research Laboratory;Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba;Criadero El Carmen;Instituto de Botánica del Nordeste ,(IBONE, CONICET-UNNE) and Facultad de Ciencias Exactas y Naturales y Agrimensura, Universidad Nacional del Nordeste;Instituto Multidisciplinario de Biología Vegetal-

关键词: Peanut Smut;    Arachis hypogaea;    Thecaphora frezii;    Peanut;    SNP genotyping;    rhAmp assay;    Genetic introgression;   

DOI  :  10.7717/peerj.10581

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Peanut smut caused by Thecaphora frezii is a severe fungal disease currently endemic to Argentina and Brazil. The identification of smut resistant germplasm is crucial in view of the potential risk of a global spread. In a recent study, we reported new sources of smut resistance and demonstrated its introgression into elite peanut cultivars. Here, we revisited one of these sources (line I0322) to verify its presence in the U.S. peanut germplasm collection and to identify single nucleotide polymorphisms (SNPs) potentially associated with resistance. Five accessions of Arachis hypogaea subsp. fastigiata from the U.S. peanut collection, along with the resistant source and derived inbred lines were genotyped with a 48K SNP peanut array. A recently developed SNP genotyping platform called RNase H2 enzyme-based amplification (rhAmp) was further applied to validate selected SNPs in a larger number of individuals per accession. More than 14,000 SNPs and nine rhAmp assays confirmed the presence of a germplasm in the U.S. peanut collection that is 98.6% identical (P < 0.01, bootstrap t-test) to the resistant line I0322. We report this germplasm with accompanying genetic information, genotyping data, and diagnostic SNP markers.

【 授权许可】

CC BY