Unraveling the genetic diversity and structure of Quercus liaotungensis population through analysis of microsatellite markers

PeerJ

article

Si Chen¹  Da Ren¹  Xinmin An²  Bin Guo²  Xiangchun Hao¹  Lijun Han¹  Yu Zhai¹  Shuai Zhou¹ 

[1] Shanxi Academy of Forestry and Grassland Sciences;Beijing Forestry University

关键词: Quercus liaotungensis Koidz.;    Genetic diversity;    Genetic structure;    Microsatellite marker;   

DOI  :  10.7717/peerj.10922

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundQuercus liaotungensis Koidz. is an ecologically and economically important tree species widely distributed in Northern China. However, the effective assessment, utilization, and protection of Q. liaotungensis resources remain unexplored.MethodsIn total, 120 samples obtained from 12 Q. liaotungensis populations of Northern China were investigated for genetic diversity and structure using 19 simple sequence repeat (SSR) primer pairs.ResultsThe total number of alleles detected was 293, the average number of effective allele (Ne) was 6.084, the genetic differentiation coefficient (Fst) was 0.033, and the mean observed heterozygosity (Ho) and expected heterozygosity (He) were 0.690 and 0.801, respectively. Moreover, analysis of molecular variance (AMOVA) showed a 5.5% genetic variation among 12 Q. liaotungensis populations, indicating that a high level of genetic diversity and a low degree of genetic differentiation among Q. liaotungensis populations. STRUCTURE and cluster analysis divided the 12 Q. liaotungensis populations into the following three subpopulations: Bashang Plateau subpopulation (SH), Liaodong Peninsula subpopulation (NC), and Loess Plateau subpopulation (other 10 populations). The cluster analysis based on 19 climatic factors was consistent with the genetic structure. A positive correlation was found between genetic distance and geographical distance (r = 0.638, p = 0.028) by the Mantel test, and two boundaries were found among the 12 Q. liaotungensis populations by the Barrier analysis, indicating that Q. liaotungensis populations existed isolated by geographical distance and physical barrier.ConclusionThis study suggests that geographical isolation, physical barrier, climatic types, and natural hybridization promote the formation of genetic structures, which can contribute to future protection and genetic improvement of Q. liaotungensis.

【 授权许可】

CC BY