High fructose exposure modifies the amount of adipocyte-secreted microRNAs into extracellular vesicles in supernatants and plasma

PeerJ

article

Adrián Hernández-Díazcouder¹  Javier González-Ramírez³  Abraham Giacoman-Martínez⁴  Guillermo Cardoso-Saldaña⁵  Eduardo Martínez-Martínez⁶  Horacio Osorio-Alonso⁷  Ricardo Márquez-Velasco²  José L. Sánchez-Gloria²  Yaneli Juárez-Vicuña²  Guillermo Gonzaga⁷  Laura Gabriela Sánchez-Lozada⁷  Julio César Almanza-Pérez⁴  Fausto Sánchez-Muñoz² 

[1] Posgrado en Biología Experimental, Universidad Autónoma Metropolitana-Iztapalapa;Departamento de Inmunología, Instituto Nacional de Cardiología Ignacio Chávez;Laboratorio de Biología Celular, Facultad de Enfermería, Universidad Autónoma de Baja California Campus Mexicali;Laboratorio de Farmacología, Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa;Departamento de Endocrinología, Instituto Nacional de Cardiología Ignacio Chávez;Laboratorio de Comunicación Celular y Vesículas Extracelulares, Instituto Nacional de Medicina Genómica;Departamento de Fisiopatología Cardio-Renal, Instituto Nacional de Cardiología Ignacio Chávez

关键词: Fructose;    Extracellular Vesicles;    Adipocytes;    Adipose tissue;    microRNA;   

DOI  :  10.7717/peerj.11305

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundHigh fructose exposure induces metabolic and endocrine responses in adipose tissue. Recent evidence suggests that microRNAs in extracellular vesicles are endocrine signals secreted by adipocytes. Fructose exposure on the secretion of microRNA by tissues and cells is poorly studied. Thus, the aim of this study was to evaluate the effect of fructose exposure on the secretion of selected microRNAs in extracellular vesicles from 3T3-L1 cells and plasma from Wistar rats.Methods3T3-L1 cells were exposed to 550 µM of fructose or standard media for four days, microRNAs levels were determined in extracellular vesicles of supernatants and cells by RT-qPCR. Wistar rats were exposed to either 20% fructose drink or tap water for eight weeks, microRNAs levels were determined in extracellular vesicles of plasma and adipose tissue by RT-qPCR.ResultsThis study showed that fructose exposure increased the total number of extracellular vesicles released by 3T3-L1 cells (p = 0.0001). The levels of miR-143-5p were increased in extracellular vesicles of 3T3-L1 cells exposed to fructose (p = 0.0286), whereas miR-223-3p levels were reduced (p = 0.0286). Moreover, in plasma-derived extracellular vesicles, miR-143-5p was higher in fructose-fed rats (p = 0.001), whereas miR-223-3p (p = 0.022), miR-342-3p (p = 0.0011), miR-140-5p (p = 0.0129) and miR-146b-5p (p = 0.0245) were lower.ConclusionFructose exposure modifies the levels of microRNAs in extracellular vesicles in vitro and in vivo. In particular, fructose exposure increases miR-143-5p, while decreases miR-223-3p and miR-342-3p.

【 授权许可】

CC BY