Key circular RNAs identified in male osteoporosis patients by whole transcriptome sequencing

PeerJ

article

Haijin Zhang¹  Xue Song²  Zongyan Teng¹  Sujun Cheng¹  Weigang Yu¹  Xiaoyi Yao¹  Zhiqiang Song¹  Yina Zhang¹ 

[1] Department of Geriatrics, The Second Affiliated Hospital of Harbin Medical University;Harbin North people’s Hospital

关键词: Whole transcriptome sequencing;    Male osteoporosis;    Non-coding RNAs;    circRNAs;   

DOI  :  10.7717/peerj.11420

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundOsteoporosis (OP) is a systemic disease with bone loss and microstructural deterioration. Numerous noncoding RNAs (ncRNAs) have been proved to participate in various diseases, especially circular RNAs (circRNAs). However, the expression profile and mechanisms underlying circRNAs in male osteoporosis have not yet been explored.MethodsThe whole transcriptome expression profile and differences in mRNAs, circRNAs, and microRNAs (miRNAs) were investigated in peripheral blood samples of patients with osteoporosis and healthy controls consisting of males ≥ 60-years-old.ResultsA total of 398 circRNAs, 51 miRNAs, and 642 mRNAs were significantly and differentially expressed in osteoporosis compared to healthy controls. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the host genes of significantly differentially expressed circRNAs were mainly enriched in the regulation of cell cycle process: biological process (BP), organelle part cellular components (CC), protein binding molecular function (MF), Toll-like receptor signaling pathway, tumor necrosis factor (TNF) signaling pathway, and thyroid hormone signaling pathway. circRNA-miRNA-mRNA regulatory network was constructed using the differentially expressed RNAs. Moreover, key circRNAs (hsa_circ_0042409) in osteoporosis were discovered and validated by qPCR.ConclusionsThe key cicrRNAs plays a major role in the pathogenesis of osteoporosis and could be used as potential biomarkers or targets in the diagnosis and treatment of osteoporosis.

【 授权许可】

CC BY