PeerJ | |
Paeoniflorin improves functional recovery through repressing neuroinflammation and facilitating neurogenesis in rat stroke model | |
article | |
Hongli Tang1  Leiruo Wu2  Xixi Chen1  Huiting Li1  Baojun Huang1  Zhenyang Huang1  Yiyang Zheng1  Liqing Zhu3  Wujun Geng1  | |
[1] Anesthesiology, the First Affiliated Hospital of Wenzhou Medical University;Endoscopy Center, the First Affiliated Hospital of Wenzhou Medical University;Clinical Laboratory, the First Affiliated Hospital of Wenzhou Medical University | |
关键词: Ischemic stroke; Paeoniflorin; Microglia; Neuroinflammation; JNK; | |
DOI : 10.7717/peerj.10921 | |
学科分类:社会科学、人文和艺术(综合) | |
来源: Inra | |
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【 摘 要 】
BackgroundMicroglia, neuron, and vascular cells constitute a dynamic functional neurovascular unit, which exerts the crucial role in functional recovery after ischemic stroke. Paeoniflorin, the principal active component of Paeoniae Radix, has been verified to exhibit neuroprotective roles in cerebralischemic injury. However, the mechanisms underlying the regulatory function of Paeoniflorin on neurovascular unit after cerebral ischemia are still unclear.MethodsIn this study, adult male rats were treated with Paeoniflorin following transient middle cerebral artery occlusion (tMCAO), and then the functional behavioral tests (Foot-fault test and modified improved neurological function score, mNSS), microglial activation, neurogenesis and vasculogenesis were assessed.ResultsThe current study showed that Paeoniflorin treatment exhibited a sensorimotor functional recovery as suggested via the Foot-fault test and the enhancement of spatial learning as suggested by the mNSS in rat stroke model. Paeoniflorin treatment repressed microglial cell proliferation and thus resulted in a significant decrease in proinflammatory cytokines IL-1β, IL-6 and TNF-α. Compared with control, Paeoniflorin administration facilitated von Willebrand factor (an endothelia cell marker) and doublecortin (a neuroblasts marker) expression, indicating that Paeoniflorin contributed to neurogenesis and vasculogenesis in rat stroke model. Mechanistically, we verified that Paeoniflorin repressed JNK and NF-κB signaling activation.ConclusionsThese results demonstrate that Paeoniflorin represses neuroinflammation and facilitates neurogenesis in rat stroke model and might be a potential drug for the therapy of ischemic stroke.
【 授权许可】
CC BY
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