PeerJ | |
Potential Arabidopsis thaliana glucosinolate genes identified from the co-expression modules using graph clustering approach | |
article | |
Sarahani Harun1  Nor Afiqah-Aleng2  Mohammad Bozlul Karim3  Altaf Ul Amin3  Shigehiko Kanaya3  Zeti-Azura Mohamed-Hussein1  | |
[1] Centre for Bioinformatics Research, Institute of Systems Biology ,(INBIOSIS), Universiti Kebangsaan Malaysia;Institute of Marine Biotechnology, Universiti Malaysia Terengganu;Graduate School of Science and Technology & NAIST Data Science Center, Nara Institute of Science and Technology;Department of Applied Physics, Faculty of Science and Technology, Universiti Kebangsaan Malaysia | |
关键词: Secondary metabolites; Nitrogen-containing compounds; Aliphatic glucosinolates; Indolic glucosinolates; Graph clustering; Gene network analysis; | |
DOI : 10.7717/peerj.11876 | |
学科分类:社会科学、人文和艺术(综合) | |
来源: Inra | |
【 摘 要 】
Background Glucosinolates (GSLs) are plant secondary metabolites that contain nitrogen-containing compounds. They are important in the plant defense system and known to provide protection against cancer in humans. Currently, increasing the amount of data generated from various omics technologies serves as a hotspot for new gene discovery. However, sometimes sequence similarity searching approach is not sufficiently effective to find these genes; hence, we adapted a network clustering approach to search for potential GSLs genes from the Arabidopsis thaliana co-expression dataset. Methods We used known GSL genes to construct a comprehensive GSL co-expression network. This network was analyzed with the DPClusOST algorithm using a density of 0.5. 0.6. 0.7, 0.8, and 0.9. Generating clusters were evaluated using Fisher’s exact test to identify GSL gene co-expression clusters. A significance score (SScore) was calculated for each gene based on the generated p-value of Fisher’s exact test. SScore was used to perform a receiver operating characteristic (ROC) study to classify possible GSL genes using the ROCR package. ROCR was used in determining the AUC that measured the suitable density value of the cluster for further analysis. Finally, pathway enrichment analysis was conducted using ClueGO to identify significant pathways associated with the GSL clusters. Results The density value of 0.8 showed the highest area under the curve (AUC) leading to the selection of thirteen potential GSL genes from the top six significant clusters that include IMDH3, MVP1, T19K24.17, MRSA2, SIR, ASP4, MTO1, At1g21440, HMT3, At3g47420, PS1, SAL1, and At3g14220. A total of Four potential genes (MTO1, SIR, SAL1, and IMDH3) were identified from the pathway enrichment analysis on the significant clusters. These genes are directly related to GSL-associated pathways such as sulfur metabolism and valine, leucine, and isoleucine biosynthesis. This approach demonstrates the ability of the network clustering approach in identifying potential GSL genes which cannot be found from the standard similarity search.
【 授权许可】
CC BY
【 预 览 】
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RO202307100005548ZK.pdf | 24253KB | download |