Analysis of genetic diversity among Chinese Cyclocybe chaxingu strains using ISSR and SRAP markers

PeerJ

article

Jin-Hao Liu¹  Fang-Hui Ding²  Hai-Yan Song¹  Ming-Hui Chen¹  Dian-Ming Hu¹ 

[1] Jiangxi Agricultural University, Bioengineering and Technological Research Centre for Edible and Medicinal Fungi;Jiangxi Agricultural University, College of Bioscience and Bioengineering;Jiangxi Key Laboratory for Conservation and Utilization of Fungal Resources, and College of Bioscience and Bioengineering;Ministry of Education of the P.R. China, Key Laboratory of Crop Physiology, Ecology and Genetic Breeding ,(Jiangxi Agricultural University)

关键词: Cyclocybe chaxingu;    Germplasm resource;    Genetic diversity;    Molecular markers;   

DOI  :  10.7717/peerj.14037

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundCyclocybe chaxingu is an edible and medicinal fungal species commonly cultivated in China. The major problems currently facing by growers of C. chaxingu is the random labeling of strains and strains aging and degeneration. Therefore, an evaluation of genetic diversity is essential for the conservation and reproducing programs of this species.MethodsIn the present study, 24 widely cultivated strains were collected from the main producing areas of China, and the genetic diversity analysis was performed. DNA polymorphism among these Chinese C. chaxingu strains was analyzed using inter-simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) markers.ResultsEight ISSR primers amplified a total of 75 DNA fragments of which 61 (81.33%) were polymorphic. Fifteen SRAP primer combinations amplified 166 fragments of which 132 (79.52%) were polymorphic. Cluster analysis showed that the C. chaxnigu strains fall into five groups with a genetic distance values ranging from 0.06 to 0.60 by ISSR analysis, while the SRAP analysis divided the test strains into four groups within the range of genetic distance from 0.03 to 0.57. The results of the present study reveal a high level of genetic diversity among the widely cultivated C. chaxingu strains.

【 授权许可】

CC BY