Anatomical and physiological responses of Aechmea blanchetiana (Bromeliaceae) induced by silicon and sodium chloride stress during in vitro culture

PeerJ

article

Mariela Mattos da Silva¹  Diolina Moura Silva¹  Andreia Barcelos Passos Lima Gontijo²  Antelmo Ralph Falqueto³  Rosiane Cipriano³  João Paulo Rodrigues Martins⁴  Lorenzo Toscano Conde² 

[1] Center for the Study of Photosynthesis, Federal University of Espírito Santo;Plant Tissue Culture Laboratory, Federal University of Espírito Santo;Plant Ecophysiology Laboratory, Federal University of Espírito Santo;Institute of Dendrology, Polish Academy of Sciences

关键词: Bromeliads;    Modulated fluorescence;    Tolerance;    Plant tissue culture;    Salinity;   

DOI  :  10.7717/peerj.14624

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Salt stress is one of the most severe abiotic stresses affecting plant growth and development. The application of silicon (Si) is an alternative that can increase the tolerance of plants to various types of biotic and abiotic stresses. The objective was to evaluate salt stress’s effect in vitro and Si’s mitigation potential on Aechmea blanchetiana plants. For this purpose, plants already established in vitro were transferred to a culture medium with 0 or 14 µM of Si (CaSiO3). After growth for 30 days, a stationary liquid medium containing different concentrations of NaCl (0, 100, 200, or 300 µM) was added to the flasks. Anatomical and physiological analyses were performed after growth for 45 days. The plants cultivated with excess NaCl presented reduced root diameter and effective photochemical quantum yield of photosystem II (PSII) (ΦPSII) and increased non-photochemical dissipation of fluorescence (qN). Plants that grew with the presence of Si also had greater content of photosynthetic pigments and activity of the enzymes of the antioxidant system, as well as higher values of maximum quantum yield of PSII (FV/FM), photochemical dissipation coefficient of fluorescence (qP) and fresh weight bioaccumulation of roots and shoots. The anatomical, physiological and biochemical responses, and growth induced by Si mitigated the effect of salt stress on the A. blanchetiana plants cultivated in vitro, which can be partly explained by the tolerance of this species to grow in sandbank (Restinga) areas.

【 授权许可】

CC BY