Sterilization Method of Etlingera Elatior Explant on Tissue Culture

3rd Annual Applied Science and Engineering Conference

工业技术;自然科学

Chaidir, L.^1 ; Isnaini, S.^2 ; Hidayat, C.^1 ; Hasani, S.^1 ; Wicaksana, N.^3 ; Syantiara, J.^1

Agrotechnology Department, Faculty of Science and Technology, UIN Sunan Gunung Djati, JL. A. H. Nasution 105, Bandung, Indonesia^1

Agrotechnology Program Study, Fakultas of Agriculture, Universitas Perjuangan Tasikmalaya, Jl. Peta 177, Tasikmalaya, Indonesia^2

Agronomy Department, Faculty of Agriculture, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang, Jatinangor, Indonesia^3

关键词: Contamination levels;    Cultivation process;    Etlingera elatior;    Infection rates;    Medicinal plants;    Plant tissue culture;    Planting materials;    Traditional medicinal plants;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/434/1/012292/pdf
DOI  :  10.1088/1757-899X/434/1/012292

来源: IOP

【 摘 要 】

Etlingera elatior or in Sundanese known as honje is a type of traditional medicinal plant that is potential to be developed. Utilization of honje raw material as a medicinal plant is still harvested in nature without cultivation process. Traditionally honje is propagated through rhizomes with high infection rates because the rhizomes experience decay caused by bacteria of Phytium species. To overcome this problem, tissue culture as a conservation effort is needed to produce free pests and diseases seedlings. This study was aimed to find out explants that gave the best results on the sterilization of honje plants that would be used as planting material on cultivation with tissue culture. This research was conducted at Plant Tissue Culture Laboratory of Agrotechnology Dept. of Uin Sunan Gunung Djati Bandung. This study used two types of explants i.e. bud and bud base in the process of sterilization, each repeated 20 times. The results showed that the best explant on honje plant on sterilization was in the treatment of base of bud explant by showing the lowest contamination level with the number of live explant almost 88% and showed the beginning of shoot formation at age 3 DAI.