Paralytic Shellfish Profiles Produced by the Toxic Dinoflagellate Pyrodinium bahamense from Sepanggar Bay, Malaysia

Pertanika Journal of Tropical Agricultural Science

article

Asilah Al-Has¹  Normawaty Mohammad-Noor¹  Sitti Raehanah M. Shaleh²  Mohd Nor Azman Ayub³  Deny Susanti¹  Ghaffur Rahim Mustakim² 

[1] Kuliyyah of Science, International Islamic University Malaysia;Borneo Marine Research Institute, Universiti Malaysia Sabah;Fisheries Research Institute

关键词: Gonyautoxin;    HPLC;    PSP;    Pyrodinium bahamense;    saxitoxin;   

DOI  :  10.47836/pjtas.46.1.20

学科分类：农业科学（综合）

来源: Universiti Putra Malaysia, Universiti Pertanian Malaysia Press

【 摘 要 】

Pyrodinium bahamense var compressum is a harmful dinoflagellate that produces saxitoxin, which causes paralytic shellfish poisoning (PSP) that is deadly to humans. A non-axenic culture of P. bahamense was established using f/2 media from samples collected from Sepanggar Bay, Kota Kinabalu, Sabah. Toxin analyses of cultures harvested on days 60, 120, 180, and 360 were performed using high-performance liquid chromatography with a fluorescence detector and compared with samples collected at the same location during the bloom in 2021. The highest cell toxin content was found in the bloom sample (86.2 fmole/cell), and no toxin was detected in the culture 60 days old. In addition, cell toxin content for the P. bahamense culture was low (9.4-16.5 fmole/cell). Based on the toxin profile, P. bahamense comprises 84- 98% of gonyautoxin 4. In summary, the current findings add to the existing knowledge of the toxin profiling of P. bahamense, a toxic, harmful algal bloom species, thus, leading to better toxin management.

【 授权许可】

Unknown