In-vitro AND In-vivo ASSESSMENT OF TOXIC EFFECTS OF Parthenium hysterophorus LEAVES EXTRACT

Journal of The Chilean Chemical Society

article

Adil Hussain¹  Ayaz Ali Khan¹  Muhammad Ali²  Ghazala Yasmin Zamani³  Zafar Iqbal⁴  Qarib Ullah⁴  Javid Iqbal¹  Muhammad Shahzad⁴  Tariq Aziz⁴ 

[1] Department of Biotechnology, University of Malakand;Department of Biotechnology, Quaid-e- Azam University Islamabad Pakistan.;Department of Biotechnology, Bacha Khan University Charsadda Pakistan.;Institute of Basic Medical Sciences, Khyber Medical University

关键词: Parthenium hysterophorus leaves;    biochemistry;    toxicity;    HPLC;    hematology;   

DOI  :  10.4067/S0717-97072022000205484

学科分类：化学（综合）

来源: Sociedad Chilena de Quimica

【 摘 要 】

Parthenium hysterophorus is an invasive plant variety found in around 50 countries. Phenolic compounds in the P. hysterophorus leaves, HPLC analysis was carried out. In addition, methanolic extract of P. hysterophorus leaves was evaluated for total phenolic content (TPC), total flavonoid content (TFC), DPPH free radical scavenging activity, and hemolytic activity. The leaves crude extract was orally administered to rabbits (n = 5) at four doses (10, 20, 40 and 80 mg kg–1) for 9 days and its effects on hematological and biochemical parameters were investigated. Statistical analysis was performed using GraphPad Prism 8.0 software. The HPLC data revealed the presence of Chlorogenic acid, Bis-HHDP-hex (pedunculagin), Morin, Ellagic acid, Rutin, Syringic acid, etc., which were detected at various retention times. Among these compounds, Ellagic acid was abundantly present with sample peak area of 9594.909 %. Total phenolic and flavonoid contents in the leaves extracts at a concentration of 80 μg were, 57.35 ± 4.12 μg GAE/μg and 39.44 ± 0.41 μg QE/μg. DPPH free radical scavenging activity was 72.82 % with IC50 value of 168 μg/μL at 80 μg of the extract. In the hemolysis assay, 200 μg of extract had highest cell inhibition of 76.90 % with IC50500. Significant (P<0.05) variation in the hematological and biochemical parameters was observed in the extract fed groups. It has been concluded that P. hysterophorus leaves extract had toxic effects on the hematological and biochemical parameters in rabbits which cause abnormal blood profile.

【 授权许可】

Unknown