The protective impact of growth hormone against rotenone-induced apoptotic cell death via acting on Endoplasmic reticulum stress and autophagy axis

Turkish Journal of Biology

article

RENCÜZOĞULLARI, ÖZGE¹  TORNACI, SELAY¹  ÇELİK, YAĞMUR¹  TAŞ, NAYAT NAROT¹  YERLİKAYA, PINAR OBAKAN¹  ARISAN, ELİF DAMLA²  GÜRKAN, AJDA ÇOKER³ 

[1] Department of Molecular Biology and Genetics, İstanbul Kültür University;Institute of Biotechnology, Gebze Technical University;Department of General Biology, Faculty of Science, Marmara University

关键词: Rotenone;    Parkinson's disease;    growth hormone;    autophagy;    endoplasmic reticulum stress;   

DOI  :  10.55730/1300-0152.2639

学科分类：生物科学（综合）

来源: Scientific and Technical Research Council of Turkey - TUBITAK

【 摘 要 】

Human growth hormone (GH) is crucial modulator of cellular metabolisms, including cell proliferation and organ development, by stimulating insulin-like growth factor-1 (IGF-1), which has various functions such as cell proliferation, tissue growth, survival, or neuroprotection. Therefore, GH is implicated as a critical player in the cell and can enhance neurogenesis and provide neuroprotection during the treatment of neurological diseases such as Parkinson's disease (PD). In this study, the neuroprotective role of GH was investigated in rotenone-induced PD models for the first time. Both SH-SY5Y and SK-N-AS neuroblastoma cells were exposed to rotenone to mimic PD pathogenesis as stated in previous studies. Our data demonstrated that overexpression of GH led to the resistance of the SH-SY5Y and SK-N-AS cell lines to rotenone treatment. The levels of ER stress markers, CHOP, PERK, XBP-1, and ATF6, were higher in wt cells than GH+ SH-SY5Y cells. However, the level of autophagy markers LC3 increased and the levels of reactive oxygen species (ROS) decreased with the overexpression of GH. Furthermore, while rotenone significantly increased the SubG1 population in the cell cycle of SH-SY5Y wt cells, there was a minor alteration in GH+ cell population. Concomitantly, the levels of the proapoptotic marker, cleaved-PARP, and positive staining of Annexin V in SH-SY5Y wt cells were higher after rotenone treatment. Together, these results revealed that overexpression of GH enhanced the autophagy response by triggering the ER stress of SH-SY5Y cells to rotenone exposure and showed a neuroprotective effect in vitro PD models.

【 授权许可】

Unknown