Scientific Standardization of Different Stem extracts of Cissus quadrangularis

RGUHS Journal of Pharmaceutical Sciences

article

Brundha U¹  VB Narayana Swamy¹  Akila E¹  Pruthvi N¹ 

[1] Department of Pharmacognosy, RR College of pharmacy

关键词: Histological examination;    Physiological evaluation;    Phytochemical screening;    Cissus quadrangularis;   

DOI  :  10.26463/rjps.12_3_7

学科分类：药学、药理学、毒理学（综合）

来源: Rajiv Gandhi University of Health Sciences

【 摘 要 】

Background: Perennial herb known as Cissus quadrangularis is found across the tropical world and has medicinal effects. It is one among the plants that is most regularly used as medicine in India.Aim: The present study was aimed to study the scientific standardization of different stem extracts of Cissus quadrangularis.Methodology: The determination of the physicochemical constants was done using powdered, shade dried Cissus quadrangularis Linn. stem material. Aqueous, chloroform, ethanol, and pet ether were used to create the solvent extracts.Results: The preliminary phytochemical screening showed presence of carbohydrates, proteins, amino acids, glycosides like saponin and flavonoids, tannins, phenols, alkaloids, and steroids. On physiological evaluation, loss of drying was found to be 6.4% W/W, extractive value of pet ether extract was found to be 3.6% W/W, chloroform extract 4.7% W/W, ethanol extract 7.2% W/W and water 8.9% W/W. The total flavonoid content of pet ether extract was found to be 19.2537±0.0632 µg/mL, chloroform extract 33.7294±0.0574 µg/mL, ethanol extract 59.8601±0.0363 µg/mL, water extract 45.6305±0.0735 µg/mL. The total phenolic content of pet ether extract was found to be 19.867±0.0565 µg/mL, chloroform extract 19.669±0.0654 µg/mL, ethanol extract 404±0.0199 µg/mL and water extract 25.658±0.0343 µg/mL. TLC of pet ether extract showed constituents having retention factor (Rf) values, chloroform extract showed constituents having Rf values 0.55, 0.46; ethanol extract showed constituents having Rf values 0.815 and 0.584; and water extract showed constituents having Rf values 0.234.Conclusion: In the current study, Cissus quadrangularis plants were examined for pharmacognostic characterization, physiochemical parameter measurement, phytochemical screening, and TLC examinations of the crude extracts Background: Perennial herb known as Cissus quadrangularis is found across the tropical world and has medicinal effects. It is one among the plants that is most regularly used as medicine in India.Aim: The present study was aimed to study the scientific standardization of different stem extracts of Cissus quadrangularis.Methodology: The determination of the physicochemical constants was done using powdered, shade dried Cissus quadrangularis Linn. stem material. Aqueous, chloroform, ethanol, and pet ether were used to create the solvent extracts.Results: The preliminary phytochemical screening showed presence of carbohydrates, proteins, amino acids, glycosides like saponin and flavonoids, tannins, phenols, alkaloids, and steroids. On physiological evaluation, loss of drying was found to be 6.4% W/W, extractive value of pet ether extract was found to be 3.6% W/W, chloroform extract 4.7% W/W, ethanol extract 7.2% W/W and water 8.9% W/W. The total flavonoid content of pet ether extract was found to be 19.2537±0.0632 µg/mL, chloroform extract 33.7294±0.0574 µg/mL, ethanol extract 59.8601±0.0363 µg/mL, water extract 45.6305±0.0735 µg/mL. The total phenolic content of pet ether extract was found to be 19.867±0.0565 µg/mL, chloroform extract 19.669±0.0654 µg/mL, ethanol extract 404±0.0199 µg/mL and water extract 25.658±0.0343 µg/mL. TLC of pet ether extract showed constituents having retention factor (Rf) values, chloroform extract showed constituents having Rf values 0.55, 0.46; ethanol extract showed constituents having Rf values 0.815 and 0.584; and water extract showed constituents having Rf values 0.234.Conclusion: In the current study, Cissus quadrangularis plants were examined for pharmacognostic characterization, physiochemical parameter measurement, phytochemical screening, and TLC examinations of the crude extracts.

【 授权许可】

CC BY