Bacillus subtilis TO-A extends the lifespan of Caenorhabditis elegans

Bioscience of Microbiota, Food and Health

article

Ryuichi SAITO¹  Naoki SATO¹  Yoichi OKINO¹  Dian-Sheng WANG¹  Genichiro SEO¹ 

[1] Bioscience R&D Department, TOA Biopharma Co., Ltd.

关键词: Clostridium butyricum;    Enterococcus faecium;    Bacillus subtilis;    Caenorhabditis elegans;    lifespan;   

DOI  :  10.12938/bmfh.2022-057

学科分类：生物科学（综合）

来源: Nihon Bifizusukin Senta / Japan Bifidus Foundation

【 摘 要 】

Clostridium butyricum TO-A, Enterococcus faecium T-110, and Bacillus subtilis TO-A are sold as oral probiotic preparations and reportedly exhibit many beneficial effects on the health of hosts, including humans and livestock. In this study, we compared the ability of these clinically applied probiotic bacteria with Escherichia coli OP50 in extending the lifespan of Caenorhabditis elegans. To compare the C. elegans lifespan-extending effects of the three bacteria, experiments were performed using a nematode growth medium containing a small amount of trypticase soy agar. The maximum lifespans of worms fed C. butyricum TO-A, E. faecium T-110, or B. subtilis TO-A increased by 11, 12, and 26%, respectively, compared with worms fed E. coli OP50. In addition, we conducted a metabolomic analysis of methanol extracts of B. subtilis TO-A cells, which exhibited the strongest lifespan-extending effect on C. elegans among the probiotic bacteria tested in this study. As a result, 59 candidate substances involved in extending the lifespan of C. elegans were identified in B. subtilis TO-A cells.

【 授权许可】

CC BY