Partial characterization of antagonistic substance produced by a Clostridium butyricum strain

Brazilian Journal of Microbiology

Nely Cristina Medeiros Caires²  Aline Magalhães Matos¹  Luiz De Macedo Farias¹  Maria Auxiliadora Roque De Carvalho¹  Regina Maria Nardi Drummond¹  Jacques Robert Nicoli¹  Antônio Paulino Ribeiro Sobrinho² 

[1] ,Universidade Federal de Minas Gerais Escola de Odontologia Departamento de Dentistica RestauradoraBelo Horizonte MG ,Brasil

关键词: Clostridium butyricum;    Fusobacterium nucleatum;    Bifidobacterium adolescentis;    antagonism;    root canal system;    Clostridium butyricum;    Fusobacterium nucleatum;    Bifidobacterium adolescentis;    antagonismo;    sistema de canais radiculares;   

DOI  :  10.1590/S1517-83822007000200014

来源: SciELO

【 摘 要 】

The production of antagonistic substance by bacterium present in the infected root canal system (RCS) probably is an important ecological factor for its successful colonization of the local. The objective of this study was to partially characterize an antagonistic substance produced by a Clostridium butyricum isolated from infected RCS.Production of inhibitory compound was evaluated by the agar double layer diffusion technique using Fusobacterium nucleatum and Bifidobacterium adolescentis as indicator bacteria. The physicochemical and biochemical factors tested for the partial characterization were influence of pH and temperature and susceptibility to the action of some proteolytic enzymes. An inhibition zone was observed against the two indicator strains and acidity and bacteriophage were rejected as responsible for this phenomenon. The inhibitory activity showed to be decreasing in a pH range from 3.5 to 6.5 and being stable at temperatures of 60º, 70º and 100ºC, but completely inactivated when exposed at 121ºC. The antagonistic activity was resistant to the proteolytic action of trypsin, a-chymotrypsin and papain. An antagonistic substance was produced by C. butyricum, which was thermo-resistant and probably of non-protein nature.

【 授权许可】

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