Antiviral activity of Bifidobacterium adolescentisSPM1005-A on human papillomavirus type 16

BMC Medicine

Research Article

Si-Won Lee¹  Hyang-Mi An¹  Nam-Joo Ha¹  Do-Kyung Lee¹  Min-Kyeong Cha¹  Kyung-Jae Kim¹  Seon-Hee Shin²  Jeong-Hyun Kwon³ 

[1] College of Pharmacy, Sahmyook University, Seoul, Republic of Korea;Department of Pediatrics, College of Medicine, Hallym University, Chuncheon, Republic of Korea;Department of Sport Medicine, Jung Won University, Chungbuk, Republic of Korea;

关键词: antiviral activity;    Bifidobacterium adolescentis;    human papillomavirus (HPV) type 16;    quantitative real-time PCR (qRT-PCR);   

DOI  :  10.1186/1741-7015-10-72

 received in 2012-04-20, accepted in 2012-07-12,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundProbiotic lactic acid bacteria (LAB) support a functional and balanced immune system, and contribute to immune modulatory effects in combatting microbial pathogens, including viruses. Most cervical cancers are associated with anogenital region infection with high-risk (HR) human papillomavirus (HPV). In this study, we analyzed the antiviral activity of Bifidobacterium adolescentis SPM1005-A in the SiHa cervical cancer cell line expressing HPV type 16.MethodsWe assessed the cellular toxicity of B. adolescentis SPM1005-A in SiHa cells by the Trypan blue dye exclusion assay. Cells (3.6 × 105) in culture plates with or without B. adolescentis SPM1005-A in the same type of medium, were incubated with HPV type 16 at a concentration of 5.1 × 107 cfu/ml. For antiviral analysis, we performed quantitative real-time PCR (qRT-PCR) for E6 and E7 oncogene expressions and observed protein levels by immunoblotting.ResultsThe qRT-PCR results showed that E6 and E7 mRNA levels decreased simultaneously. Western blot analysis revealed that the E6 protein expression slightly decreased after 24 and 48 h, but the level of E7 protein expression appear unaffected compared with that in the control. Decreased HPV16 E6 and E7 mRNA transcript and protein levels were not associated with cell morphology or significant cytotoxic effects.ConclusionsThis study showed that B. adolescentis SPM1005-A had antiviral activity through suppression E6 and E7 oncogene expression. The results suggest that B. adolescentis SPM1005-A could be potential applications of HPV-associated cervical cancer prevention.

【 授权许可】

Unknown   
© Cha et al; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.